MTA3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MTA3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Kat-Nr. : 14682-1-AP

Synonyme

Metastasis-associated protein MTA3, MTA 3, mta1

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  WB analysis using 14682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14682-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 14682-1-AP

  IP result of anti-MTA3 (IP:14682-1-AP, 4ug; Detection:14682-1-AP 1:3000) with Raji cells lysate 2840 ug.

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  IHC staining of mouse brain using 14682-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14682-1-AP (MTA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14682-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14682-1-AP (MTA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 14682-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (14682-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U-251 using 14682-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (14682-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maus-Thymusgewebe, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 10 publications below
• IP: See 1 publications below

Produktinformation

14682-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MTA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTA3 fusion protein Ag6400
• Vollständiger Name: metastasis associated 1 family, member 3
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 62 kDa, 68 kDa
• GenBank-Zugangsnummer: BC053631
• Gene symbol: MTA3
• Gene ID (NCBI): 57504
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.

Publikationen

Species	Application	Title
mouse	WB	Cell Stem Cell; The polycomb group protein L3mbtl2 assembles an atypical PRC1-family complex that is essential in pluripotent stem cells and early development.; Authors - Jinzhong Qin
mouse		Mol Cell; The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression.; Authors - Susanne Bornelöv
human	IHC	Oncotarget; Metastasis-associated protein 3 in colorectal cancer determines tumor recurrence and prognosis.; Authors - Yi Huang
human	WB,IHC	PLoS One; Overexpression of MTA3 Correlates with Tumor Progression in Non-Small Cell Lung Cancer.; Authors - Li Haiying H ; KD Validated
human	IHC	J Gastroenterol Hepatol; Overexpression of the metastasis-associated gene MTA3 correlates with tumor progression and poor prognosis in hepatocellular carcinoma.; Authors - Chuanxi Wang
human	WB,IHC	Tumour Biol; MTA3 regulates malignant progression of colorectal cancer through Wnt signaling pathway.; Authors - Taiwei Jiao