MTCH2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MTCH2 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Rezensionen (1)

Kat-Nr. : 16888-1-AP

Synonyme

MIMP, Met-induced mitochondrial protein, HSPC032

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  WB analysis using 16888-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16888-1-AP (MTCH2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16888-1-AP

  IP result of anti-MTCH2 (IP:16888-1-AP, 3ug; Detection:16888-1-AP 1:1000) with HepG2 cells lysate 4000ug.

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  IHC staining of human liver using 16888-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16888-1-AP (MTCH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16888-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16888-1-AP (MTCH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat testis using 16888-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat testis tissue using MTCH2 antibody (16888-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat testis using 16888-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat testis tissue using MTCH2 antibody (16888-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U-251 using 16888-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTCH2 antibody (16888-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenhodengewebe
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:14000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 21 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• CoIP: See 2 publications below

Produktinformation

16888-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA MTCH2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTCH2 fusion protein Ag10399
• Vollständiger Name: mitochondrial carrier homolog 2 (C. elegans)
• Berechnetes Molekulargewicht: 303 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC000875
• Gene symbol: MTCH2
• Gene ID (NCBI): 23788
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology; Authors - Gayathri Muthukumar ; KD Validated
	WB	Sci Adv; ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution; Authors - Michael J McKenna
human	WB,IHC	Cell Death Dis; MTCH2 regulates NRF2-mediated RRM1 expression to promote melanoma proliferation and dacarbazine insensitivity; Authors - Xuedan Zhang ; KD Validated
human	WB,IF	J Cell Biol; The modified mitochondrial outer membrane carrier MTCH2 links mitochondrial fusion to lipogenesis.; Authors - Katherine Labbé
pig	WB	FASEB J; MTCH2 promotes adipogenesis in intramuscular preadipocytes via an m6A-YTHDF1-dependent mechanism.; Authors - Qin Jiang
human	WB	RNA Biol; Reprogramming of m6A epitranscriptome is crucial for shaping of transcriptome and proteome in response to hypoxia.; Authors - Yan-Jie Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xianhe (Verified Customer) (06-23-2022)

WB band is strong and clear, IFA image binding great.

• Applications: Western Blot, Immunofluorescence