MTERF Polyklonaler Antikörper

MTERF Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 16957-1-AP

Synonyme

MTERF, MTERF1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 16957-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16957-1-AP (MTERF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16957-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16957-1-AP (MTERF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

16957-1-AP bindet in WB,ELISA MTERF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTERF fusion protein Ag10640
• Vollständiger Name: mitochondrial transcription termination factor
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC000965
• Gene symbol: MTERF
• Gene ID (NCBI): 7978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mitochondrial transcription termination factor (MTERF)also named as Mterf1 is a 399 amino acid protein, which localizes in the mitochondrion and belongs to the mTERF family. MTERF contains three leucine zippers that form a three-stranded coiled-coil that binds to DNA. It has been suggested that only the phosphorylated form of MTERF has transcription termination activity. MTERF plays a central role in the control of mitochondrial rRNA and mRNA synthesis. MTERFD1 is also thought to act as a mitochondrial transcription regulator and is expressed as two isoforms produced by alternative splicing.Using DNA affinity chromatography, a fraction called MTERF was found to be associated with foot-printing capacity, and this contained two polypeptides of 34 and 31 kDa. Moreover, termination-promoting activity appears to reside in the 34-kDa protein. (PMID:7681833).

Publikationen

Species	Application	Title
human	WB	Nat Immunol; Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.; Authors - Jing Geng
mouse	WB	Nat Commun; Mice lacking the mitochondrial exonuclease MGME1 accumulate mtDNA deletions without developing progeria.; Authors - Stanka Matic