MTF1 Polyklonaler Antikörper

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• KD/KO Validated

MTF1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, IP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(36)

Rezensionen (1)

Kat-Nr. : 25383-1-AP

Synonyme

MTF 1, Transcription factor MTF 1, Transcription factor MTF-1, ZRF

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• 

  WB analysis of mouse liver using 25383-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25383-1-AP (MTF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 25383-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25383-1-AP (MTF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 25383-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25383-1-AP (MTF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25383-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25383-1-AP (MTF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25383-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25383-1-AP (MTF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 25383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25383-1-AP (MTF1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 25383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25383-1-AP (MTF1 Antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Jurkat-Zellen, Maushirngewebe, Mausherzgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 27 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 5 publications below

Produktinformation

25383-1-AP bindet in WB, IHC, IF, IP, chIP, ELISA MTF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Fisch, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTF1 fusion protein Ag21848
• Vollständiger Name: metal-regulatory transcription factor 1
• Berechnetes Molekulargewicht: 753 aa, 81 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa, 100 kDa
• GenBank-Zugangsnummer: BC014454
• Gene symbol: MTF1
• Gene ID (NCBI): 4520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Metal regulatory transcription factor 1 (MTF1), contains six C2H2-type zinc finger and localizes in the nucleus. MTF1 is a 753 amino acid protein and calculated molecular weight is 81 kDa. We always dectected a 65-70 protein by western blot. MTF1 binds to the metal responsive element and activates the metallothionein I promoter.

Publikationen

Species	Application	Title
mouse,human	WB	Cancer Cell; Hypoxia inducible factor-1α drives cancer resistance to cuproptosis; Authors - Zhou Yang
human	WB	Adv Sci (Weinh); LncRNA MT1DP Aggravates Cadmium-Induced Oxidative Stress by Repressing the Function of Nrf2 and is Dependent on Interaction with miR-365.; Authors - Ming Gao ; KD Validated
human	WB	Nat Commun; Autophagy of the m6A mRNA demethylase FTO is impaired by low-level arsenic exposure to promote tumorigenesis.; Authors - Yan-Hong Cui ; KD Validated
fish	WB,IP	Environ Sci Technol; Zn Induces Lipophagy via the Deacetylation of Beclin1 and Alleviates Cu-Induced Lipotoxicity at Their Environmentally Relevant Concentrations.; Authors - Xiaolei Wei
	WB	Environ Sci Technol; Mitochondria-Dependent Oxidative Stress Mediates ZnO Nanoparticle (ZnO NP)-Induced Mitophagy and Lipotoxicity in Freshwater Teleost Fish.; Authors - Guang-Hui Chen
human	WB,IHC	Oxid Med Cell Longev; Ferroportin-Dependent Iron Homeostasis Protects against Oxidative Stress-Induced Nucleus Pulposus Cell Ferroptosis and Ameliorates Intervertebral Disc Degeneration In Vivo.; Authors - Saideng Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

David (Verified Customer) (09-15-2025)

Concentration used 1:1000. Works pretty good. Strong clean signal

• Applications: Western Blot