MTMR2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MTMR2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 14372-1-AP

Synonyme

CMT4B, CMT4B1, KIAA1073, MTMR2, myotubularin related protein 2

---

• 

  WB analysis of HeLa using 14372-1-AP

  WB result of MTMR2 antibody (14372-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR2 transfected HeLa cells.

• 

  WB analysis using 14372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 14372-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 14372-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse cerebellum using 14372-1-AP

  IP result of anti-MTMR2 (IP:14372-1-AP, 4ug; Detection:14372-1-AP 1:300) with mouse cerebellum tissue lysate 4000 ug.

• 

  IHC staining of human endometrial cancer using 14372-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 14372-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 14372-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cerebellum using 14372-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Cerebellum-Gewebe, NIH/3T3-Zellen
• Erfolgreiche IP: Maus-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe, humanes Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14372-1-AP bindet in WB, IP, IHC, ELISA MTMR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTMR2 fusion protein Ag5745
• Vollständiger Name: myotubularin related protein 2
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 66-73 kDa
• GenBank-Zugangsnummer: BC052990
• Gene symbol: MTMR2
• Gene ID (NCBI): 8898
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTMR2(myotubularin-related protein 2) is also named as KIAA1073 and belongs to the non-receptor class myotubularin subfamily, which is characterized by the presence of a phosphatase domain. It may regulate vesicular trafficking in Schwann cells (PMID:17880751) and its reversible phosphorylation represents a critical mechanism for regulating the subcellular localization of MTMR2(PMID:21372139). MTMR2 is a 73 kDa protein in its monomeric form and exist as a dimer within cells and the protein re-localizes to membranes in hypo-osmotic settings(PMID:16399794). Defects in MTMR2 are the cause of Charcot-Marie-Tooth disease type 4B1 (CMT4B1). MTMR2 has two isofroms with the molecular mass of 73 and 66 kDa.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kincaid (Verified Customer) (05-21-2024)

This product worked as expected. I completed a GFP-nanotrap IP (Chromotek agarose beads) and ran 2% whole cell lysate with IP fraction on an SDS-PAGE gel. Resolved proteins were transferred to a PVDF membrane and probed with aMTMR2. GFP-CCDC22 is known to pull-down MTMR2 and is validated with this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

MTMR2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14372-1-AP)