MTPN Polyklonaler Antikörper

MTPN Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13508-1-AP

Synonyme

GCDP, MTPN, myotrophin, Protein V 1, V 1

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Galerie der Validierungsdaten

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  WB analysis using 13508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13508-1-AP (MTPN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13508-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13508-1-AP (MTPN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human cerebellum using 13508-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 13508-1-AP (MTPN Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 13508-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13508-1-AP (MTPN Antibody) at dilution of 1:50.

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  IHC staining of human heart using 13508-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13508-1-AP (MTPN Antibody) at dilution of 1:50.

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  IHC staining of human cerebellum using 13508-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 13508-1-AP (MTPN Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 13508-1-AP

  Immunofluorescent analysis of HepG2 cells, using MTPN antibody 13508-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 13508-1-AP

  Immunofluorescent analysis of Hela cells, using MTPN antibody 13508-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, humanes Hirngewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Cerebellum-Gewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

13508-1-AP bindet in WB, IHC, IF, ELISA MTPN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTPN fusion protein Ag4419
• Vollständiger Name: myotrophin
• Berechnetes Molekulargewicht: 118 aa, 13 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC028093
• Gene symbol: MTPN
• Gene ID (NCBI): 136319
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myotrophin (MTPN) is a ubiquitously expressed 12 kDa cytoplamic protein that was first isolated from the hearts of spontaneously hypertensive rats. Protein sequence analysis revealed that MTPN was composed of 118 amino acids and was occupied by two and a half internal 33 amino acid ankyrin repeats. MTPN stimulates protein synthesis and increases the expression of a number of cardiac marker genes (such as atrial natriuretic factor and b-myosin heavy chain) in cardiomyocytes leading to hypertrophy. In skeletal muscle, MTPN is also a positive growth factor in promoting skeletal muscle growth.

Publikationen

Species	Application	Title
human	IHC	Am J Obstet Gynecol; Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression.; Authors - Roni Levy
human	IHC	J Clin Biochem Nutr; Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction.; Authors - Masataka Nomoto