MTRF1L Polyklonaler Antikörper

MTRF1L Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16694-1-AP

Synonyme

MRF1L, mtRF1a, MTRF1L

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 16694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16694-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16694-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16694-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16694-1-AP (MTRF1L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

16694-1-AP bindet in WB, ELISA MTRF1L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTRF1L fusion protein Ag10081
• Vollständiger Name: mitochondrial translational release factor 1-like
• Berechnetes Molekulargewicht: 380 aa, 44 kDa
• Beobachtetes Molekulargewicht: 40-46 kDa
• GenBank-Zugangsnummer: BC011873
• Gene symbol: MTRF1L
• Gene ID (NCBI): 54516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTRF1L, also named as Peptide chain release factor 1-like, mitochondrial, is a 380 amino acid protein, which belongs to the prokaryotic/mitochondrial release factor family and locates in the mitochondrial. MTRF1L directs the termination of translation in response to the peptide chain termination codons UAA and UAG. MTRF1L is expressed in skeletal muscle.

Publikationen

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice.; Authors - Takumi Yokokawa
mouse	WB	Physiol Rep; Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.; Authors - Takumi Yokokawa
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li