MTX2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MTX2 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11610-1-AP

Synonyme

metaxin 2, MTX2

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 11610-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11610-1-AP (MTX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse kidney using 11610-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11610-1-AP (MTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11610-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11610-1-AP (MTX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse heart using 11610-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11610-1-AP (MTX2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse heart using 11610-1-AP

  IP Result of anti-MTX2 (IP:11610-1-AP, 3ug; Detection:11610-1-AP 1:300) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 11610-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11610-1-AP (MTX2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11610-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11610-1-AP (MTX2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11610-1-AP

  Immunofluorescent analysis of HepG2 cells, using MTX2 antibody 11610-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mausnierengewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

11610-1-AP bindet in WB, IP, IHC, IF, ELISA MTX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTX2 fusion protein Ag2181
• Vollständiger Name: metaxin 2
• Berechnetes Molekulargewicht: 263 aa, 30 kDa
• Beobachtetes Molekulargewicht: 25-29 kDa
• GenBank-Zugangsnummer: BC017271
• Gene symbol: MTX2
• Gene ID (NCBI): 10651
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Rep; Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.; Authors - Fenglei Jian ; KD Validated
human	WB	Cell Death Dis; EB virus-induced ATR activation accelerates nasopharyngeal carcinoma growth via M2-type macrophages polarization.; Authors - Bo Zhang
mouse	WB	Hepatology; Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury; Authors - Li Chen
human	WB	Cell Death Differ; Sam50-Mic19-Mic60 axis determines mitochondrial cristae architecture by mediating mitochondrial outer and inner membrane contact; Authors - Junhui Tang
mouse	IF	J Ovarian Res; Proteomic analysis implicates that postovulatory aging leads to aberrant gene expression, biosynthesis, RNA metabolism and cell cycle in mouse oocytes; Authors - Chuanxin Zhang
mouse	WB	Cell Rep; Mitochondrial cristae architecture protects against mtDNA release and inflammation; Authors - Baiyu He ; KO Validated