CDHR5 Polyklonaler Antikörper

CDHR5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 25619-1-AP

Synonyme

Cadherin-related family member 5, MLPCDH, MUCDHL, Mucin and cadherin-like protein, Mucin-like protocadherin

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  WB analysis of HEK-293 using 25619-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25619-1-AP (MUPCDH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25619-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human kidney using 25619-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human small intestine using 25619-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 25619-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25619-1-AP (MUPCDH Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

25619-1-AP bindet in WB, IHC, ELISA CDHR5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CDHR5 fusion protein Ag22537
• Vollständiger Name: mucin-like protocadherin
• Berechnetes Molekulargewicht: 845 aa, 88 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC038405
• Gene symbol: MUPCDH
• Gene ID (NCBI): 53841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MUPCDH (Mu-protocadherin), also known as MUCDHL (Mucin and cadherin-like protein) and CDHR5 (Cadherin-related family member 5), is a member of the cadherin superfamily. It is involved in calcium-dependent cell adhesion and may have additional functions in terminally differentiated epithelia. The MUPCDH gene maps to 11p15.5, with highest expression in kidney, liver, colon and small intestine. Alternatively spliced transcript variants encoding different isoforms have been described. (PMID: 12167596; 21839066)

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis; Authors - Yesheng Fu