MX1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MX1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (6)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

86

Rezensionen (1)

Kat-Nr. : 13750-1-AP

Synonyme

IFI 78K, IFI78, MX, MX1, MxA, Myxovirus resistance protein 1

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Galerie der Validierungsdaten

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  WB analysis using 13750-1-AP

  Lysates of A549 cells treated or non-treated with IFN-alpha were subjected to SDS PAGE followed by western blot with 13750-1-AP (MX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13750-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13750-1-AP (MX1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 cells using 13750-1-AP

  IP result of anti-MX1 (13750-1-AP for IP and Detection) with A549 cell.

• 

  IHC staining of human tonsillitis using 13750-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13750-1-AP (MX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13750-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 13750-1-AP (MX1 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 13750-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 13750-1-AP (MX1 Antibody) at dilution of 1:50.

• 

  IF Staining of A549 using 13750-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MX1 antibody (13750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A549 using 13750-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human MX1 (13750-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Mauslungengewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen
• Erfolgreiche Detektion in FC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 67 publications below
• IHC: See 9 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Produktinformation

13750-1-AP bindet in WB, IP, IHC, IF, FC, ELISA MX1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, hamster, Hausschwein, Huhn, Hund, Maus, Makake
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MX1 fusion protein Ag4692
• Vollständiger Name: Myxovirus resistance protein 1
• Berechnetes Molekulargewicht: 662 aa, 76 kDa
• Beobachtetes Molekulargewicht: 76 kDa
• GenBank-Zugangsnummer: BC032602
• Gene symbol: MX1
• Gene ID (NCBI): 4599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN. This antibody can recognize the endogenous Mx1 protein in several tissues like lung and spleen.

Publikationen

Species	Application	Title
human	WB	Nature; IFITM3 restricts the morbidity and mortality associated with influenza.; Authors - Everitt AR
human	WB	Cell Host Microbe; NRAV, a long noncoding RNA, modulates antiviral responses through suppression of interferon-stimulated gene transcription.; Authors - Jing Ouyang
human		Nat Commun; Large-scale and high-resolution mass spectrometry-based proteomics profiling defines molecular subtypes of esophageal cancer for therapeutic targeting.; Authors - Wei Liu
human	WB	Hepatology; MxA inhibits hepatitis B virus replication by interaction with hepatitis B core antigen.; Authors - Li Ning N
macaque	IHC	Nat Commun; Repeated semen exposure decreases cervicovaginal SIVmac251 infection in rhesus macaques.; Authors - Shaheed A Abdulhaqq
human	WB	J Hepatol; PMID: 25481566; Authors - Shi Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hannah (Verified Customer) (09-21-2020)

Used for western blot on human fibroblasts -/+ interferon treatment, detected single band only in IFN treated samples.

• Applications: Western Blot
• Primary Antibody Dilution: 1/500

MX1 Antibody Western Blot validation (1/500 dilution) in (Cat no:13750-1-AP)

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