MX1 Polyklonaler Antikörper

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• KD/KO Validated

MX1 Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

Rezensionen (1)

Kat-Nr. : CL488-13750

Synonyme

IFI 78K, IFI78, Interferon induced protein p78, MX, MX1, MxA, Myxovirus resistance protein 1

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  FC experiment of A549 using CL488-13750

  1X10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human MX1 (CL488-13750) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): A549-Zellen
• Erfolgreiche Detektion in FC: A549-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

CL488-13750 bindet in FC (Intra) MX1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MX1 fusion protein Ag4692
• Vollständiger Name: Myxovirus resistance protein 1
• Berechnetes Molekulargewicht: 662 aa, 76 kDa
• Beobachtetes Molekulargewicht: 76 kDa
• GenBank-Zugangsnummer: BC032602
• Gene symbol: MX1
• Gene ID (NCBI): 4599
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN. This antibody can recognize the endogenous Mx1 protein in several tissues like lung and spleen.

Publikationen

Species	Application	Title
mouse	FC	Small; A Synthetic Multivalent Lipopeptide Derived from Pam3CSK4 with Irreversible Influenza Inhibition and Immuno-Stimulating Effects; Authors - Yong Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alexandra (Verified Customer) (03-01-2024)

The antibody works very well, on a low concentration and gives a very nice signal.

• Applications: Flow Cytometry