MYBPC1 Polyklonaler Antikörper

MYBPC1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 22900-1-AP

Synonyme

C-protein, skeletal muscle slow isoform, MYBPCS, Myosin-binding protein C, slow-type, Slow MyBP C, Slow MyBP-C

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  WB analysis of mouse skeletal muscle using 22900-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22900-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 22900-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22900-1-AP (MYBPC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22900-1-AP (MYBPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 22900-1-AP (MYBPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22900-1-AP (MYBPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human bladder using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bladder using 22900-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 22900-1-AP (MYBPC1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Blasengewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Maus-Blasengewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

22900-1-AP bindet in WB, IHC, ELISA MYBPC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin binding protein C, slow type
• Berechnetes Molekulargewicht: 128 kDa
• Beobachtetes Molekulargewicht: 135-140 kDa
• GenBank-Zugangsnummer: NM_002465
• Gene symbol: MYBPC1
• Gene ID (NCBI): 4604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYBPC1, also named as MYBPCS, belongs to the immunoglobulin superfamily and MyBP family. It is a thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. This antibody is specific to MYBPC1.

Publikationen

Species	Application	Title
mouse,human	IHC	Mol Psychiatry; FXR1 regulation of parvalbumin interneurons in the prefrontal cortex is critical for schizophrenia-like behaviors.; Authors - Minjie Shen
rat	WB	Dokl Biochem Biophys; Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?; Authors - I O Petrova
mouse	IHC	Adv Biol (Weinh); Glucose-Responsive Fibrin Hydrogel-Based Multimodal Nucleic Acid Delivery System; Authors - Mangesh Morey

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sirsendu (Verified Customer) (06-19-2020)

Used for immunohistochemistry of db/db mice skin tissue after wound healing.Please provide amazon UK gift (£20)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mice skin tissue