MYC tag Polyklonaler Antikörper
MYC tag Polyklonal Antikörper für IF, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
rekombinanten Protein und mehr (3)
Anwendung
WB, IP, IHC, IF, FC, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 16286-1-AP
Synonyme
"MYC tag Antibodies" Comparison
View side-by-side comparison of MYC tag antibodies from other vendors to find the one that best suits your research needs.
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Rekombinantes Protein Protein, Recombinant protein |
| Erfolgreiche IP | Transfizierte HEK-293-Zellen |
| Erfolgreiche Detektion in IF | Transfizierte HEK-293-Zellen, MCF-7-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunfluoreszenz (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 197 publications below |
| IHC | See 1 publications below |
| IF | See 31 publications below |
| IP | See 64 publications below |
| FC | See 3 publications below |
| CoIP | See 30 publications below |
Produktinformation
16286-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA MYC tag und zeigt Reaktivität mit rekombinanten Protein
| Getestete Reaktivität | rekombinanten Protein |
| In Publikationen genannte Reaktivität | human, Huhn, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | MYC tag fusion protein Ag9409 |
| Vollständiger Name | MYC tag |
| Gene symbol | |
| Gene ID (NCBI) | |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL.
What is the molecular weight of myc?
1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code).
What are the applications for myc tag?
The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106).
What is the structure of myc tag?
The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for MYC tag antibody 16286-1-AP | Protokoll herunterladen |
| IF protocol for MYC tag antibody 16286-1-AP | Protokoll herunterladen |
| IP protocol for MYC tag antibody 16286-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Nat Microbiol Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry. | ||
Nat Commun TRIM5α recruits HDAC1 to p50 and Sp1 and promotes H3K9 deacetylation at the HIV-1 LTR | ||
Mol Cell CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity | ||
Mol Cell Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells. | ||
Nat Struct Mol Biol ARF GTPases activate Salmonella effector SopF to ADP-ribosylate host V-ATPase and inhibit endomembrane damage-induced autophagy. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Nazarine (Verified Customer) (02-01-2024) | Works well for Western Blot, IF, IP
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FH Nazarine (Verified Customer) (02-01-2024) | Works well for Western Blot and IF and IP
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FH Amy (Verified Customer) (03-05-2023) | Detected overexpressed Mac-tagged proteins in HEK293T cell lysates but with a fair few background bands.
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FH James (Verified Customer) (11-30-2022) | Very good antibody
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FH Boyan (Verified Customer) (08-03-2022) | gives a good signal in IF
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FH Chahat (Verified Customer) (06-08-2022) | The myc antibody gives clear staining!
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FH WEI (Verified Customer) (03-08-2022) | Good antibody for detecting overexpression proteins in 293 cells.
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FH Minze (Verified Customer) (01-11-2022) | it works well for immunofluorecence so far.
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FH Phoebe (Verified Customer) (03-01-2019) | This antibody was very clean in a western blot to detect Myc-tagged pol2 in S cerevisiae whole cell extract and chromatin fractionation samples.
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