MYC tag Monoklonaler Antikörper

MYC tag Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

rekombinanten Protein

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Publikationen(1)

CloneNo.

1A5A2

Rezensionen (1)

Kat-Nr. : CL647-60003

Synonyme

EQKLISEEDL

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  IF Staining of Transfected HEK-293 using CL647-60003

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647-conjugated MYC tag antibody (CL647-60003, Clone: 1A5A2 ) at dilution of 1:800.

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  FC experiment of Transfected HEK-293 using CL647-60003

  1X10^6 Transfected HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-MYC tag (CL647-60003, Clone:1A5A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HEK-293 transfected with GFP-MYC using CL647-60003

  1x10^6 HEK-293 transfected with GFP-MYC were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated MYC tag Monoclonal antibody (CL647-60003, Clone:1A5A2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): Transfizierte HEK-293-Zellen, Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-60003 bindet in IF/ICC, FC (Intra) MYC tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Myc tag
• Gene symbol: Myc tag
• Gene ID (NCBI): 99
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Publikationen

Species	Application	Title
		Biochem Pharmacol; Peptide-based CAR-NK cells: A novel strategy for the treatment of solid tumors; Authors - Qianqian Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lisbeth (Verified Customer) (10-20-2023)

Used for detecting myc-tag labelled proteins on cell surfaces, even after fixation.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1:50