MYC tag Monoklonaler Antikörper

MYC tag Monoklonal Antikörper für WB, IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, IF/ICC

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

1A5A2

Kat-Nr. : CL750-60003

Synonyme

myc, EQKLISEEDL

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  WB analysis using CL750-60003

  HEK-293 cell lysates and Recombinant protein(Ag19172) were subjected to SDS PAGE followed by western blot with CL750-60003 (MYC tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected HEK-293 using CL750-60003

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 750 MYC tag antibody (CL750-60003, Clone: 1A5A2 ) at dilution of 1:100.

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  FC experiment of HEK-293 transfected with GFP-MYC using CL750-60003

  1x10^6 HEK-293 transfected with GFP-MYC were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated MYC tag Monoclonal antibody (CL750-60003, Clone:1A5A2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche Detektion in IF/ICC: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL750-60003 bindet in WB, IF/ICC MYC tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Myc tag
• Gene symbol: Myc tag
• Gene ID (NCBI): 99
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.