MYH1 Monoklonaler Antikörper

MYH1 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

CloneNo.

1G10H9

Rezensionen (1)

Kat-Nr. : 67299-1-Ig

Synonyme

1G10H9, MyHC-2x, MyHC-IIx/d, Myosin 1, Myosin-1

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  WB analysis of human skeletal muscle using 67299-1-Ig

  1 μg human skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skeletal muscle using 67299-1-Ig

  1 μg pig skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 67299-1-Ig

  1 μg mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 67299-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 67299-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 67299-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:80000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 67299-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYH1 antibody (67299-1-Ig, Clone: 1G10H9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe, Hausschwein-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:2000-1:20000
• Immunfluoreszenz (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

67299-1-Ig bindet in WB, IHC, IF-P, ELISA MYH1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Kaninchen, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYH1 fusion protein Ag17129
• Vollständiger Name: myosin, heavy chain 1, skeletal muscle, adult
• Berechnetes Molekulargewicht: 1939 aa, 223 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC114545
• Gene symbol: MYH1
• Gene ID (NCBI): 4619
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

Publikationen

Species	Application	Title
pig	WB	BMC Genomics; Identification of different myofiber types in pigs muscles and construction of regulatory networks; Authors - Chenchen Li
pig	WB	Food Funct; Potential nutritional healthy-aging strategy: enhanced protein metabolism by balancing branched-chain amino acids in a finishing pig model.; Authors - Lingyu Zhang
human	IHC	Pathol Res Pract; Layer-specific proteomic profiling of human normal heart; Authors - Yu Kakimoto
mouse	IF	Nutrients; Sodium Butyrate Alleviates Heat Stress-Induced Oxidative Stress and Skeletal Muscle Homeostasis Disruption by Promoting Autophagy in Mice; Authors - Jiayin Lu
rabbit	IHC	Invest Ophthalmol Vis Sci; One-Month-Old Rabbits Exhibit a Longer Postoperative Remodeling in Extraocular Muscles Compared to 3-Month-Old Rabbits; Authors - Jiangtao Lou
mouse	WB	J Muscle Res Cell Motil; RNA-seq and ChIP-seq unveils thyroid hormone receptor α deficiency affects skeletal muscle myoblast proliferation and differentiation via Col6a1 during aging; Authors - Runqing Shi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Moon (Verified Customer) (10-10-2022)

normal tissue-1/2 (N1/N2) tumor tissue-1/2/3 (T1/T2/T3)

• Applications: Western Blot
• Primary Antibody Dilution: 1:100000
• Cell Tissue Type: renal carcinoma cells

MYH1 Antibody Western Blot validation (1:100000 dilution) in renal carcinoma cells (Cat no:67299-1-Ig)