SMMHC Monoklonaler Antikörper

SMMHC Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

5C6D3

Kat-Nr. : 60222-1-Ig

Synonyme

MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain

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Galerie der Validierungsdaten

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  WB analysis using 60222-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60222-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ileum using 60222-1-Ig

  human ileum tissue were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60222-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast hyperplasia using 60222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 60222-1-Ig

  Immunofluorescent analysis of HeLa cells using 60222-1-Ig (SMMHC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Kaninchen-Magengewebe, HeLa-Zellen, humanes Ileumgewebe, Ratten-Rektumgewebe
• Erfolgreiche Detektion in IHC: humanes Mammahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 4 publications below

Produktinformation

60222-1-Ig bindet in WB, IHC, IF, ELISA SMMHC und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMMHC fusion protein Ag13397
• Vollständiger Name: myosin, heavy chain 11, smooth muscle
• Berechnetes Molekulargewicht: 1938 aa, 224 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC101677
• Gene symbol: MYH11
• Gene ID (NCBI): 4629
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.

Publikationen

Species	Application	Title
rat	IF	Biochim Biophys Acta Mol Basis Dis; Methamphetamine induces thoracic aortic aneurysm/dissection through C/EBPβ.; Authors - Bao-Ying Luo
mouse	WB,IF	Atherosclerosis; Nrf2 deficiency attenuates atherosclerosis by reducing LOX-1-mediated proliferation and migration of vascular smooth muscle cells.; Authors - Hongliang Li
rat	WB	Nutr Metab Cardiovasc Dis; Effects of carotid baroreceptor stimulation on aortic remodeling in obese rats.; Authors - Qiao Yu
mouse	IF,WB	Int J Mol Sci; Dietary Evodiamine Inhibits Atherosclerosis-Associated Changes in Vascular Smooth Muscle Cells; Authors - Yiwen Zha
human,mouse	WB,IF	Mol Cell Biochem; HRD1 reduction promotes cholesterol-induced vascular smooth muscle cell phenotypic change via endoplasmic reticulum stress; Authors - Linli Wang

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