Myosin Light Chain 2/MLC-2V Polyklonaler Antikörper
Myosin Light Chain 2/MLC-2V Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte, Zebrafisch und mehr (2)
Anwendung
WB, IP, IHC, IF, FC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10906-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Mausherzgewebe, Rattenherzgewebe |
| Erfolgreiche IP | Mausherzgewebe |
| Erfolgreiche Detektion in IHC | Mausherzgewebe, humanes Herzgewebe, Rattenherzgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF | Mausherzgewebe |
| Erfolgreiche Detektion in FC | C2C12-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:400-1:1600 |
| Immunfluoreszenz (IF) | IF : 1:50-1:500 |
| Durchflusszytometrie (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 53 publications below |
| IHC | See 9 publications below |
| IF | See 122 publications below |
| FC | See 11 publications below |
Produktinformation
10906-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Myosin Light Chain 2/MLC-2V und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch
| Getestete Reaktivität | human, Maus, Ratte, Zebrafisch |
| In Publikationen genannte Reaktivität | human, Affe, Hausschwein, Maus, Ratte, Zebrafisch |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Myosin Light Chain 2/MLC-2V fusion protein Ag1356 |
| Vollständiger Name | myosin, light chain 2, regulatory, cardiac, slow |
| Berechnetes Molekulargewicht | 19 kDa |
| Beobachtetes Molekulargewicht | 19 kDa |
| GenBank-Zugangsnummer | BC015821 |
| Gene symbol | MYL2 |
| Gene ID (NCBI) | 4633 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Background
MYL2 belongs to the myosin family of motor proteins. Their common features are ATP hydrolysis, actin binding, and potential for kinetic energy transduction. MYL2 stands for Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC-2), also known as the regulatory light chain of myosin (RLC). This isoform is distinct from those expressed in skeletal muscle (MYLPF), smooth muscle (MYL12B), and cardiac atrial muscle (MYL7) (PMID: 21345328). Due to its tissue specificity, MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
What is the molecular weight of MYL2?
There are two isoforms of this protein in humans that differ only slightly in length and produce proteins composed of 152 and 166 aa, which correspond to about 19 kDa (PMID: 16678204).
What is the subcellular localization of MYL2?
It is uniquely expressed in the cytoplasm of heart muscles. It colocalizes with actin cytoskeleton staining.
What is the tissue specificity of MYL2?
MYL2 is specifically expressed in ventricular cardiac tissue.
What is the function of MYL2 in cardiac tissue?
MYL2 is a contractile protein that plays a role in heart development and function. During cardiogenesis it plays an early role in cardiac contractility by promoting cardiac myofibril assembly and represents one of the earliest markers of ventricular specification (PMID 8506363).
In general, MYL2 interacts with the neck/tail region of the muscle thick filament protein myosin to regulate myosin motility and function (PMID 8316858). Its N-terminal domain is responsible for calcium and magnesium binding at their activating concentrations. This induces a functionally important conformational change (PMID: 18202317). However, the ion dissociation rate is not fast enough to modulate cardiac contractility on a beat-by-beat basis (PMIDs: 188447, 8804617). In addition, RLC function can be modulated by posttranslational modifications such as phosphorylation and deamidation in the N-terminal region. As a result, a significant change in the charge of the protein is introduced, which undoubtedly alters its interaction with the C-terminal myosin alpha-helical domain (PMID: 26074085).
What is MYL2's involvement in disease?
Diseases associated with MYL2 dysfunction include forms of familial hypertrophic cardiomyopathies, congenital fiber-type disproportion, and heart failure (PMID: 26074085).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-AP | Protokoll herunterladen |
| IHC protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-AP | Protokoll herunterladen |
| IF protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-AP | Protokoll herunterladen |
| IP protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Science Conversion of human fibroblasts into functional cardiomyocytes by small molecules. | ||
Cell Stem Cell Cardiac repair in a porcine model of acute myocardial infarction with human induced pluripotent stem cell-derived cardiovascular cells. | ||
Cell Stem Cell Pathogenic LMNA variants disrupt cardiac lamina-chromatin interactions and de-repress alternative fate genes. | ||
Cell Stem Cell Gene editing to prevent ventricular arrhythmias associated with cardiomyocyte cell therapy | ||
Cell Stem Cell Gene editing to prevent ventricular arrhythmias associated with cardiomyocyte cell therapy | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Udesh (Verified Customer) (12-29-2023) | Worked well for WB at 1:1000 in Glioma cells
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FH Brice-Emmanuel (Verified Customer) (12-18-2023) | Works very well in WB, even after more than 10 days at room temperature due to a delivery problem.
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FH Rebecca (Verified Customer) (10-28-2022) | 20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.
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FH WEI (Verified Customer) (05-11-2022) | Strong, specific band for heart tissues
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FH Irem (Verified Customer) (04-19-2022) | This antibody unspecifically recognizes at least four proteins in Jurkat cell lysate. The specific band, around 17 kDa, is so faint.
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FH Ning (Verified Customer) (02-20-2022) | This antibody works very well in immunofluorescence staining for iPSC-derived ventricular cardiomyocytes.
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FH Tongbin (Verified Customer) (08-25-2020) | This MLC2 antibody works very well in western blots.
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