Myosin Light Chain 2/MLC-2V Polyklonaler Antikörper

Myosin Light Chain 2/MLC-2V Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 55462-1-AP

Synonyme

MLC 2v, MYL2, MLC 2, MLC2, MLC-2

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  WB analysis of rat heart using 55462-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 55462-1-AP (MYL2 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55462-1-AP

  IP result of anti-Myosin Light Chain 2/MLC-2V (IP:55462-1-AP, 4ug; Detection:55462-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 55462-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55462-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 55462-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55462-1-AP (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 55462-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55462-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55462-1-AP (MYL2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of C2C12 using 55462-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myosin Light Chain 2/MLC-2V (55462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenherzgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

55462-1-AP bindet in WB, IHC, IF, FC (Intra), IP, ELISA Myosin Light Chain 2/MLC-2V und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, light chain 2, regulatory, cardiac, slow
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: NM_000432
• Gene symbol: Myosin Light Chain 2
• Gene ID (NCBI): 4633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Immunol; Sexual dimorphism of lung immune-regulatory units imprint biased pulmonary fibrosis; Authors - Peng Xiang
mouse	IF	Stem Cell Reports; Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4.; Authors - Kiwon Ban
human	WB	iScience; SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury.; Authors - Anteneh Getachew
human	WB	Int J Mol Med; Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis.; Authors - Chao Ci