MYL3 Monoklonaler Antikörper

MYL3 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2E4B2

Kat-Nr. : 66286-1-Ig

Synonyme

Cardiac myosin light chain 1, CMH8, CMLC1, MLC1SB, MLC1V, MYL3, Myosin light chain 3, VLC1

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  WB analysis using 66286-1-Ig

  Various tissue lysates were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 66286-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 66286-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 66286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens).

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  IHC staining of human heart using 66286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 66286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 66286-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, humanes Herzgewebe, Mausherzgewebe, Hausschwein-Herzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• RIP: See 1 publications below

Produktinformation

66286-1-Ig bindet in WB, IHC, RIP, ELISA MYL3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MYL3 fusion protein Ag24535
• Vollständiger Name: myosin, light chain 3, alkali; ventricular, skeletal, slow
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa
• GenBank-Zugangsnummer: BC009790
• Gene symbol: MYL3
• Gene ID (NCBI): 4634
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

Publikationen

Species	Application	Title
human	WB	Antioxidants (Basel); Ganoderma lucidum Spore Powder Alleviates Metabolic-Associated Fatty Liver Disease by Improving Lipid Accumulation and Oxidative Stress via Autophagy; Authors - Yuxuan Zhang
mouse	WB,RIP	Research (Wash D C); Circ-0001283 Aggravates Cardiac Hypertrophy by Targeting Myosin Light Chain 3 Protein; Authors - Wenjing Wang
human	WB	Transl Oncol; ACAT1 suppresses clear cell renal cell carcinoma progression by AMPK mediated fatty acid metabolism; Authors - Ming Zheng