MYOM1-Specific Polyklonaler Antikörper

MYOM1-Specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 20360-1-AP

Synonyme

MYOM1, MYOM1-Specific, Myomesin 1, myomesin 1, 185kDa, Myomesin family member 1, SKELEMIN

---

Galerie der Validierungsdaten

• 

  WB analysis using 20360-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 20360-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of C2C12 using 20360-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cell using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 20360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 20360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 20360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 20360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Skelettmuskelgewebe, Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Produktinformation

20360-1-AP bindet in WB, RIP, IHC, IF, ELISA MYOM1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myomesin 1, 185kDa
• Berechnetes Molekulargewicht: 188 kDa
• Beobachtetes Molekulargewicht: 190 kDa
• GenBank-Zugangsnummer: NM_003803
• Gene symbol: MYOM1
• Gene ID (NCBI): 8736
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYOM1 is a major component of the vertebrate myofibrillar M band. MYOM1 binds myosin, titin, and light meromyosin. This binding is dose-dependent. The antibody is specific to MYOM1.

Publikationen

Species	Application	Title
mouse	WB	Sci Rep; Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.; Authors - Catarina Rippe
mouse	WB	J Cell Mol Med; Hydrogen sulphide ameliorating skeletal muscle atrophy in db/db mice via Muscle RING finger 1 S-sulfhydration.; Authors - Fangping Lu
mouse	WB,RIP	iScience; Splicing factor Srsf5 deletion disrupts alternative splicing and causes noncompaction of ventricular myocardium; Authors - Xiaoli Zhang, Ze
rat	IHC,IF	Cell Signal; Long non-coding RNA Pvt1 modulates the pathological cardiac hypertrophy via miR-196b-mediated OSMR regulation.; Authors - Qingqing Wu
rat	IF	Int J Mol Sci; Skeletal Muscle Dysfunction in Experimental Pulmonary Hypertension; Authors - Kosmas Kosmas