Methylmalonyl Coenzyme A mutase/MUT Rekombinanter Antikörper

Methylmalonyl Coenzyme A mutase/MUT Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242198F11

Kat-Nr. : 84879-5-RR

Synonyme

MUT, 242198F11, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase

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  WB analysis using 84879-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84879-5-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 84879-5-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84879-5-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 84879-5-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84879-5-RR (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A431 using 84879-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Methylmalonyl Coenzyme A mutase/MUT Recombinant antibody (84879-5-RR, Clone:242198F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84879-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84879-5-RR against Human Methylmalonyl Coenzyme A mutase/MUT were performed. The affinity constant is 1.83 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Maushirngewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84879-5-RR bindet in WB, IHC, FC (Intra), ELISA Methylmalonyl Coenzyme A mutase/MUT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Methylmalonyl Coenzyme A mutase/MUT fusion protein Ag10523
• Vollständiger Name: methylmalonyl Coenzyme A mutase
• Berechnetes Molekulargewicht: 750 aa, 83 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC016282
• Gene symbol: MUT
• Gene ID (NCBI): 4594
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA. This condition can cause severe health issues including developmental delays, metabolic acidosis, and neurological problems. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine.