Mouse IgG2a isotype control Monoklonaler Antikörper

Mouse IgG2a isotype control Monoklonal Antikörper für WB, FC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Megathura crenulata

Anwendung

WB, FC , IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

11A1B2

Rezensionen (1)

Kat-Nr. : 66360-2-Ig

Synonyme

Mouse IgG2a

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• Validation Data Gallery
• Proteintech vs. Other Vendors

• 

  WB analysis using 66360-2-Ig

  Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of human PBMCs using 66360-2-Ig

  1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG2a isotype control Monoclonal antibody (66360-2-Ig, Clone:11A1B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or unstained (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IP: See 1 publications below
• FC: See 1 publications below

Produktinformation

66360-2-Ig bindet in WB, FC , IP, ELISA Mouse IgG2a isotype control und zeigt Reaktivität mit Megathura crenulata

• Getestete Reaktivität: Megathura crenulata
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Mouse IgG2a isotype control
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This antibody is used as the isotype control of mouse IgG2a. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Publikationen

Species	Application	Title
	FC	Biosens Bioelectron; Spherical nucleic acids-based cascade signal amplification for highly sensitive detection of exosomes.; Authors - Lei Wang
	IP	Nature; CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1; Authors - Milan Hluchý
		Nat Commun; Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire; Authors - Thomas Eden
		Plant Biotechnol J; ZmHSFA2B self-regulatory loop is critical for heat tolerance in maize; Authors - Nannan Song
		J Vasc Interv Radiol; Ethiodized Oil Pickering Emulsion for Sustained Release of Anti-PD-L1 Antibodies; Authors - Hiroki Satomura

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Niklas (Verified Customer) (07-31-2023)

better staining control than secondary only control

• Applications: Immunofluorescence, Flow Cytometry
• Primary Antibody Dilution: 1:200-1:400
• Cell Tissue Type: mouse and human heart