MSI2 Polyklonaler Antikörper

MSI2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10770-1-AP

Synonyme

MSI2, MSI2H, Musashi 2, musashi homolog 2 (Drosophila)

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Galerie der Validierungsdaten

• 

  WB analysis of PC-12 using 10770-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10770-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10770-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10770-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10770-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10770-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10770-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10770-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10770-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10770-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody) at dilution of 1:500.

• 

  IHC staining of human ovary tumor using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10770-1-AP (MSI2 antibody) at dilution of 1:10 (under 10x lens).

• 

  IHC staining of human gliomas using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10770-1-AP (MSI2 antibody) at dilution of 1:10 (under 10x lens).

• 

  IHC staining of human gliomas using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10770-1-AP (MSI2 antibody) at dilution of 1:10 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, C6-Zellen, COLO 320-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

10770-1-AP bindet in WB, IP, IHC, IF, ELISA MSI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MSI2 fusion protein Ag1213
• Vollständiger Name: musashi homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC001526
• Gene symbol: MSI2
• Gene ID (NCBI): 124540
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MSI2 is an RNA-binding protein that regulates the expression of target mRNAs at the translation level. It binds to RNA via 2 ribonucleoprotein-type RNA recognition motifs in their N-termini, to consensus motifs in mRNAs to inhibit transcription. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. MSI2 can regulate normal hematopoiesis and promote aggressive myeloid leukemia

Publikationen

Species	Application	Title
human	WB,IF	Science; Neurodevelopmental protein Musashi-1 interacts with the Zika genome and promotes viral replication.; Authors - Pavithra L Chavali
human	WB	EMBO J; Suppression of miR-199a maturation by HuR is crucial for hypoxia-induced glycolytic switch in hepatocellular carcinoma.; Authors - Ling-Fei Zhang
human	WB	Int J Oncol; Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.; Authors - Wei Jia
human	IHC	Mod Pathol; Progenitor stem cell marker expression by pulmonary carcinomas.; Authors - Moreira Andre L AL
human	WB, IP, IF	Front Mol Biosci; RNA-Binding Protein MSI2 Binds to miR-301a-3p and Facilitates Its Distribution in Mitochondria of Endothelial Cells.; Authors - Qian Qian Guo
human	IHC	Exp Cell Res; Msi2 plays a carcinogenic role in esophageal squamous cell carcinoma via regulation of the Wnt/β-catenin and Hedgehog signaling pathways.; Authors - Zhiwei Li