NAB2 Polyklonaler Antikörper

NAB2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 19601-1-AP

Synonyme

Protein MADER, NGFI-A-binding protein 2, Nab 2, Melanoma-associated delayed early response protein, MADER

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  WB analysis of human heart using 19601-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 19601-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 19601-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19601-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19601-1-AP (NAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 19601-1-AP

  IP result of anti-NAB2 (IP:19601-1-AP, 4ug; Detection:19601-1-AP 1:700) with K-562 cells lysate 3200ug.

• 

  IHC staining of human brain using 19601-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 19601-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 19601-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19601-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19601-1-AP (NAB2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 19601-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NAB2 antibody (19601-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, humanes Hirngewebe, K-562-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

19601-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NAB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NAB2 fusion protein Ag6346
• Vollständiger Name: NGFI-A binding protein 2 (EGR1 binding protein 2)
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC065931
• Gene symbol: NAB2
• Gene ID (NCBI): 4665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Invest Ophthalmol Vis Sci; β1-Integrin Deletion From the Lens Activates Cellular Stress Responses Leading to Apoptosis and Fibrosis.; Authors - Yichen Wang
mouse	WB	Lab Anim (NY); Early growth response 2 in the mPFC regulates mouse social and cooperative behaviors; Authors - Yanli Zhang