NAC1 Polyklonaler Antikörper

NAC1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 14551-1-AP

Synonyme

NACC1, Nucleus accumbens-associated protein 1, NAC-1, BTBD14B, BTB/POZ domain-containing protein 14B

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  WB analysis using 14551-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14551-1-AP (NAC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14551-1-AP

  IP result of anti-NAC1 (IP:14551-1-AP, 4ug; Detection:14551-1-AP 1:300) with MCF-7 cells lysate 3200ug.

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  IHC staining of mouse ovary using 14551-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14551-1-AP (NAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 14551-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14551-1-AP (NAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14551-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14551-1-AP (NAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14551-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14551-1-AP (NAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 14551-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14551-1-AP (NAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 14551-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using NAC1 antibody (14551-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maus-Eierstockgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

14551-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NAC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NAC1 fusion protein Ag6096
• Vollständiger Name: nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC055396
• Gene symbol: NACC1
• Gene ID (NCBI): 112939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NACC1, also named as BTBD14B and NAC1, belongs to the BTB/POZ family. NACC1 is a transcriptional repressor, which is amplified and overexpressed in various human cancers and plays critical roles in tumor development, progression, and drug resistance (PMID: 31101655). NACC1 has 1 isoform with the molecular mass of 57 kDa. While NAC1 has been shown to repress the transcription of two tumor suppressors, Gadd45-g and Gadd45-g- interacting protein 1, scientists have found that NACC1 also plays an important role in nontranscriptional function, for example, it can act as a bridge for MAVS and TBK1 in RLR-mediated signaling (PMID: 31235549).

Publikationen

Species	Application	Title
human	WB	Oncol Rep; lncRNA FOXP4‑AS1 predicts poor prognosis and accelerates the progression of mantle cell lymphoma through the miR‑423‑5p/NACC1 pathway.; Authors - Hong-Fang Tao
human	WB	J Clin Transl Hepatol; Exosome-transported circHDAC1_004 Promotes Proliferation, Migration, and Angiogenesis of Hepatocellular Carcinoma by the miR-361-3p/NACC1 Axis; Authors - Bin Xu
human	WB	Genes Genomics; ST6GALNAC1 promotes the invasion and migration of breast cancer cells via the EMT pathway; Authors - Yunzhao Luo