NAP1L4 Polyklonaler Antikörper

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• KD/KO Validated

NAP1L4 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 16018-1-AP

Synonyme

hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2

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  WB analysis of HeLa using 16018-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16018-1-AP

  WB result of NAP1L4 antibody (16018-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L4 transfected HeLa cells.

• 

  WB analysis of A431 using 16018-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16018-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16018-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16018-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16018-1-AP

  IP result of anti-NAP1L4 (IP:16018-1-AP, 4ug; Detection:16018-1-AP 1:1000) with HeLa cells lysate 4000ug.

• 

  IHC staining of human liver using 16018-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16018-1-AP (NAP1L4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16018-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16018-1-AP (NAP1L4 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HEK-293-Zellen, Mauslebergewebe, Maushodengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Produktinformation

16018-1-AP bindet in WB, IHC, IF, IP, RIP, ELISA NAP1L4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NAP1L4 fusion protein Ag8827
• Vollständiger Name: nucleosome assembly protein 1-like 4
• Berechnetes Molekulargewicht: 375 aa, 43 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC022090
• Gene symbol: NAP1L4
• Gene ID (NCBI): 4676
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Cell Biol; Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.; Authors - Chen Chen
human	WB	Mol Cell Proteomics; A novel immuno-competitive capture mass spectrometry strategy for protein-protein interaction profiling reveals that LATS kinases regulate HCV replication through NS5A phosphorylation.; Authors - Hélène Meistermann
mouse	IF,WB	J Virol; NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation.; Authors - Francisco Dominguez
human	WB,IHC	J Dermatol Sci; Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells.; Authors - Satoru Mizuhashi ; KD Validated
human	WB	Nat Struct Mol Biol; Mechanism of client selection by the protein quality-control factor UBE2O; Authors - Matthew C J Yip
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi