Napsin A Polyklonaler Antikörper

Napsin A Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16558-1-AP

Synonyme

NAPSA, Asp 4, ASP4, Aspartyl protease 4, EC:3.4.23.-

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  WB analysis of mouse lung using 16558-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16558-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16558-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16558-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16558-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16558-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16558-1-AP (Napsin A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16558-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16558-1-AP (Napsin A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16558-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16558-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 16558-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Napsin A antibody (16558-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A549 using 16558-1-AP

  1x10^6 A549 cells were intracellularly stained with 4 ug Napsin A Polyclonal antibody (16558-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 4.00 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16558-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Napsin A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Napsin A fusion protein Ag9786
• Vollständiger Name: napsin A aspartic peptidase
• Berechnetes Molekulargewicht: 420 aa, 45 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC017842
• Gene symbol: Napsin A
• Gene ID (NCBI): 9476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).