NARFL-Specific Polyklonaler Antikörper

NARFL-Specific Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 20209-1-AP

Synonyme

CIAO3, HPRN, IOP1, LET1L, NARFL, NARFL-Specific, PRN, Protein related to Narf

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 20209-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 20209-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20209-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20209-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 20209-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 20209-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 20209-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

20209-1-AP bindet in WB, IHC, ELISA NARFL-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nuclear prelamin A recognition factor-like
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 26-28 kDa
• GenBank-Zugangsnummer: NM_022493
• Gene symbol: NARFL
• Gene ID (NCBI): 64428
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NARFL, also named as PRN and IOP1, belongs to the NARF family. NAFL is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. It is required for maturation of extramitochondrial Fe/S proteins. It seems to negatively regulate the level of HIF1A expression, although this effect could be indirect. [18270200], [16956324] The antibody is specific to NARFL.

Publikationen

Species	Application	Title
human	WB	Free Radic Biol Med; The iron-sulfur cluster assembly network component NARFL Is a key element in the cellular defense against oxidative stress.; Authors - Monique Corbin