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  • KD/KO Validated

NAT10 Polyklonaler Antikörper

NAT10 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(72)

Kat-Nr. : 13365-1-AP

Synonyme

18S rRNA cytosine acetyltransferase, ALP, EC:2.3.1.-, KIAA1709, N acetyltransferase 10



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen
Erfolgreiche IPHEK-293-Zellen, Maushodengewebe
Erfolgreiche Detektion in IHChuman ovary cancer tissue, humanes Magenkrebsgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCNIH/3T3-Zellen, HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:20000-1:100000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13365-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA NAT10 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Hund, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NAT10 fusion protein Ag4184
Vollständiger Name N-acetyltransferase 10 (GCN5-related)
Berechnetes Molekulargewicht 1025 aa, 116 kDa
Beobachtetes Molekulargewicht 116 kDa
GenBank-ZugangsnummerBC035558
Gene symbol NAT10
Gene ID (NCBI) 55226
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. NAT10 is the first enzyme to be found to catalyze ac4C production in eukaryotic RNA and has acetyltransferase activity and RNA-binding activity. (PMID: 23592795). NAT10 is the singular human enzyme to have both acetyltransferase and RNA binding activities (PMID: 30449621). NAT10 is associated with U3 small nucleolar RNA and acetylates upstream binding factors to activate rRNA transcription (PMID: 21177859). NAT10 promoted cisplatin chemoresistance in bladder cancer cells by enhancing DNA damage repair (DDR) (PMID: 36939377). NAT10 is a vital member of the Gcn5-related N-acetyltransferase (GNAT) family and contains 1,025 amino acids with a molecular weight of 116 kDa (PMID: 37128278). NAT10 is commonly expressed in lymphoid tissue, kidney, liver, cerebellum, cerebral cortex, and the central nervous system during the embryonic period. Normal tissue cells express NAT10 in the nucleus, while tumor cells translocate NAT10 to the cytoplasm, nucleoplasm, and nuclear membrane, affecting tumor formation (PMID: 37128278). The specificity of this antibody has been tested by siRNA. (24786082)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for NAT10 antibody 13365-1-APProtokoll herunterladen
IHC protocol for NAT10 antibody 13365-1-APProtokoll herunterladenl
IF protocol for NAT10 antibody 13365-1-APProtokoll herunterladen
IP protocol for NAT10 antibody 13365-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IP

Cell

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

Authors - Daniel Arango
  • KO Validated
humanWB,IF

Science

Chemical inhibition of NAT10 corrects defects of laminopathic cells.

Authors - Delphine Larrieu
  • KD Validated
humanWB,IP

Nat Commun

N-acetyltransferase 10 is implicated in the pathogenesis of cycling T cell-mediated autoimmune and inflammatory disorders in mice

Authors - Wen-Ping Li
humanWB

Cell Host Microbe

Acetylation of Cytidine Residues Boosts HIV-1 Gene Expression by Increasing Viral RNA Stability.

Authors - Kevin Tsai
  • KO Validated
humanWB

Mol Cell

Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.

Authors - Daniel Arango
canineWB

Nucleic Acids Res

N4-acetylcytidine coordinates with NP1 and CPSF5 to facilitate alternative RNA processing during the replication of minute virus of canines

Authors - Xueyan Zhang
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

SONAM (Verified Customer) (12-18-2023)

Antibody works really well. No Nonspecific bindings.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: cancer cellls
FH

Mattia (Verified Customer) (09-12-2019)

Clear band at the right size, when antibody used at 1:1500 dilution in 5% milk an incubated at 2hrs at 25C. Band is a bit streaky, may require further optimisation.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: HeLa, HEK293T
FH

Jonathan (Verified Customer) (11-29-2018)

  • Applications: Western Blot, Immunofluorescence,