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NAT10 Polyklonaler Antikörper
NAT10 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (3)
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 13365-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A549-Zellen, HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen |
Erfolgreiche IP | HEK-293-Zellen, Maushodengewebe |
Erfolgreiche Detektion in IHC | human ovary cancer tissue, humanes Magenkrebsgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | NIH/3T3-Zellen, HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:20000-1:100000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
Produktinformation
13365-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA NAT10 und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Hund, Maus, Ratte, Zebrafisch |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | NAT10 fusion protein Ag4184 |
Vollständiger Name | N-acetyltransferase 10 (GCN5-related) |
Berechnetes Molekulargewicht | 1025 aa, 116 kDa |
Beobachtetes Molekulargewicht | 116 kDa |
GenBank-Zugangsnummer | BC035558 |
Gene symbol | NAT10 |
Gene ID (NCBI) | 55226 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. NAT10 is the first enzyme to be found to catalyze ac4C production in eukaryotic RNA and has acetyltransferase activity and RNA-binding activity. (PMID: 23592795). NAT10 is the singular human enzyme to have both acetyltransferase and RNA binding activities (PMID: 30449621). NAT10 is associated with U3 small nucleolar RNA and acetylates upstream binding factors to activate rRNA transcription (PMID: 21177859). NAT10 promoted cisplatin chemoresistance in bladder cancer cells by enhancing DNA damage repair (DDR) (PMID: 36939377). NAT10 is a vital member of the Gcn5-related N-acetyltransferase (GNAT) family and contains 1,025 amino acids with a molecular weight of 116 kDa (PMID: 37128278). NAT10 is commonly expressed in lymphoid tissue, kidney, liver, cerebellum, cerebral cortex, and the central nervous system during the embryonic period. Normal tissue cells express NAT10 in the nucleus, while tumor cells translocate NAT10 to the cytoplasm, nucleoplasm, and nuclear membrane, affecting tumor formation (PMID: 37128278). The specificity of this antibody has been tested by siRNA. (24786082)
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for NAT10 antibody 13365-1-AP | Protokoll herunterladen |
IHC protocol for NAT10 antibody 13365-1-AP | Protokoll herunterladenl |
IF protocol for NAT10 antibody 13365-1-AP | Protokoll herunterladen |
IP protocol for NAT10 antibody 13365-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
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Nat Commun N-acetyltransferase 10 is implicated in the pathogenesis of cycling T cell-mediated autoimmune and inflammatory disorders in mice | ||
Cell Host Microbe Acetylation of Cytidine Residues Boosts HIV-1 Gene Expression by Increasing Viral RNA Stability.
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Mol Cell Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine. | ||
Nucleic Acids Res N4-acetylcytidine coordinates with NP1 and CPSF5 to facilitate alternative RNA processing during the replication of minute virus of canines
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Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH SONAM (Verified Customer) (12-18-2023) | Antibody works really well. No Nonspecific bindings.
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FH Mattia (Verified Customer) (09-12-2019) | Clear band at the right size, when antibody used at 1:1500 dilution in 5% milk an incubated at 2hrs at 25C. Band is a bit streaky, may require further optimisation.
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FH Jonathan (Verified Customer) (11-29-2018) |
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