• Featured Product
  • KD/KO Validated

Nucleolin/C23 Polyklonaler Antikörper

Nucleolin/C23 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(52)

Kat-Nr. : 10556-1-AP

Synonyme

C23, nucleolin, FLJ45706, NCL, Protein C23



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, Rattenhirngewebe
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Lungenkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:500-1:1500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10556-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA Nucleolin/C23 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Nucleolin/C23 fusion protein Ag0859
Vollständiger Name nucleolin
Berechnetes Molekulargewicht 76 kDa
Beobachtetes Molekulargewicht 100-110 kDa
GenBank-ZugangsnummerBC006494
Gene symbol NCL
Gene ID (NCBI) 4691
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Nucleolin/C23 antibody 10556-1-APProtokoll herunterladen
IHC protocol for Nucleolin/C23 antibody 10556-1-APProtokoll herunterladenl
IF protocol for Nucleolin/C23 antibody 10556-1-APProtokoll herunterladen
IP protocol for Nucleolin/C23 antibody 10556-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nature

C9orf72 nucleotide repeat structures initiate molecular cascades of disease.

Authors - Aaron R Haeusler
humanWB

Mol Cell

Aberrant phase separation drives membranous organelle remodeling and tumorigenesis

Authors - Xinyu Wang
ratIF

Neuron

Antisense Proline-Arginine RAN Dipeptides Linked to C9ORF72-ALS/FTD Form Toxic Nuclear Aggregates that Initiate In Vitro and In Vivo Neuronal Death.

Authors - Xinmei Wen
mouseWB

Genome Res

The nuclear matrix protein HNRNPU maintains 3D genome architecture globally in mouse hepatocytes.

Authors - Hui Fan
humanWB

Clin Transl Med

LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing

Authors - Qi Wen Chen
  • KD Validated
mouseWB

Protein Cell

Nuclear m6A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos.

Authors - Chuan Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HEK293T for Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293T
{{ptg:RelatedPrimaryAntibodies}}