NCL Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NCL Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

33

Rezensionen (1)

Kat-Nr. : 10556-1-AP

Synonyme

C23, NCL, nucleolin, Protein C23

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Galerie der Validierungsdaten

• 

  WB analysis using 10556-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10556-1-AP (NCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10556-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10556-1-AP (NCL Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  IP experiment of HeLa using 10556-1-AP

  IP Result of anti-NCL (IP:10556-1-AP, 4ug; Detection:10556-1-AP 1:1000) with HeLa cells lysate 3200ug.

• 

  IHC staining of human prostate cancer using 10556-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10556-1-AP (NCL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10556-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10556-1-AP (NCL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10556-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10556-1-AP (NCL Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10556-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10556-1-AP (NCL Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 10556-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HEK-293 using 10556-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HEK-293 using 10556-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NCL antibody (10556-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10556-1-AP

  1X10^6 HeLa cells were stained with 0.20ug NCL antibody (10556-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 12 publications below
• IP: See 3 publications below

Produktinformation

10556-1-AP bindet in WB, IP, IHC, IF, FC, ELISA NCL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCL fusion protein Ag0859
• Vollständiger Name: nucleolin
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC006494
• Gene symbol: NCL
• Gene ID (NCBI): 4691
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)

Publikationen

Species	Application	Title
human	WB	Nature; C9orf72 nucleotide repeat structures initiate molecular cascades of disease.; Authors - Aaron R Haeusler
rat	IF	Neuron; Antisense Proline-Arginine RAN Dipeptides Linked to C9ORF72-ALS/FTD Form Toxic Nuclear Aggregates that Initiate In Vitro and In Vivo Neuronal Death.; Authors - Xinmei Wen
mouse	WB	Genome Res; The nuclear matrix protein HNRNPU maintains 3D genome architecture globally in mouse hepatocytes.; Authors - Hui Fan
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen ; KD Validated
mouse	WB	Protein Cell; Nuclear m6A reader YTHDC1 regulates the scaffold function of LINE1 RNA in mouse ESCs and early embryos.; Authors - Chuan Chen
human,mouse	IF	EMBO J; The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.; Authors - Ella Doron-Mandel

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HEK293T for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T

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