NDEL1 Polyklonaler Antikörper

NDEL1 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17262-1-AP

Synonyme

EOPA, MITAP1, Mitosin associated protein 1, NDEL1, NUDEL, Protein Nudel

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Galerie der Validierungsdaten

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  WB analysis of human brain using 17262-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17262-1-AP (NDEL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17262-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17262-1-AP (NDEL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17262-1-AP

  IP Result of anti-NDEL1 (IP:17262-1-AP, 4ug; Detection:17262-1-AP 1:300) with HepG2 cells lysate 2400ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

17262-1-AP bindet in WB, IP, IF, ELISA NDEL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDEL1 fusion protein Ag10975
• Vollständiger Name: nudE nuclear distribution gene E homolog (A. nidulans)-like 1
• Berechnetes Molekulargewicht: 345 aa, 38 kDa, 41 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa
• GenBank-Zugangsnummer: BC026101
• Gene symbol: NDEL1
• Gene ID (NCBI): 81565
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear distribution factor E-homolog 1 (NDE1), NDE-like 1 (NDEL1), and Lissencephaly 1 (LIS1) together involve in essential neurodevelopmental processes, including neuronal precursor proliferation and differentiation, neurite outgrowth, and neuronal migration. All three bind directly to Disrupted in Schizophrenia 1 (DISC1), a scaffold protein critical to neuronal proliferation, integration, migration, and synaptic function within the developing and adult brain.[PMID: 18469341] Nuclear distribution element-like 1 (NDEL1) was firstly identified as a regulator of the cytoskeleton in microtubule and intermediate fliament dynamics and microtubule-based transport [PMID:21948775]. It is required for organization of the cellular microtubule array and microtubule anchoring at the centrosome, which is in part by targeting the microtubule severing protein KATNA1 to the centrosome[PMID:15728732].

Publikationen

Species	Application	Title
mouse	WB,IF	J Cell Biol; Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis.; Authors - Sumigray Kaelyn D KD
mouse	IP, WB	Elife; Sequential phosphorylation of NDEL1 by the DYRK2-GSK3β complex is critical for neuronal morphogenesis.; Authors - Youngsik Woo
human	WB,IF	Sci Rep; Regulation of the actin cytoskeleton by the Ndel1-Tara complex is critical for cell migration.; Authors - Ji-Ho Hong
human	WB	Ann Neurol; Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.; Authors - Hyowon Hong
mouse	WB	Proc Natl Acad Sci U S A; Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule-actin crosstalk during neurodevelopment; Authors - Dong Jin Mun