NDRG2 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

NDRG2 Rekombinant Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241039E8

Kat-Nr. : 83902-7-RR

Synonyme

241039E8, NDRG family member 2, N-myc downstream-regulated gene 2 protein, Protein NDRG2, Protein Syld709613

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  WB analysis using 83902-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83902-7-RR (NDRG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 83902-7-RR

  WB result of NDRG2 antibody (83902-7-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDRG2 transfected HepG2 cells.

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  IHC staining of mouse brain using 83902-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83902-7-RR (NDRG2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83902-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83902-7-RR (NDRG2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 83902-7-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NDRG2 Recombinant antibody (83902-7-RR, Clone:241039E8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83902-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83902-7-RR against Human NDRG2 were performed. The affinity constant is 0.114 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen, humanes Herzgewebe, Mausherzgewebe, Rattenhirngewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83902-7-RR bindet in WB, IHC, FC (Intra), ELISA NDRG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NDRG2 fusion protein Ag2629
• Vollständiger Name: NDRG family member 2
• Berechnetes Molekulargewicht: 371 aa, 39 kDa
• Beobachtetes Molekulargewicht: 41-45 kDa
• GenBank-Zugangsnummer: BC010458
• Gene symbol: NDRG2
• Gene ID (NCBI): 57447
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

N-myc downstream-regulated gene 2 (NDRG2) is a newly identified member of the NDRG family that is highly localized and plays an important role in the regulation of astrocyte function. In astrocytes, NDRG2 affects the regulation of apoptosis, astrogliosis, blood-brain barrier integrity, and glutamate clearance. Several preclinical studies have revealed that NDRG2 is implicated in the pathogenesis of many neurological diseases, such as stroke, neurodegeneration and psychiatric disorders. Recent studies demonstrated that NDRG2 functions as a tumor suppressor and may be a prognostic predictor for many different malignant tumors.