NEDD4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NEDD4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(61)

Rezensionen (2)

Kat-Nr. : 21698-1-AP

Synonyme

Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, EC:2.3.2.26, HECT-type E3 ubiquitin transferase NEDD4, KIAA0093

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  WB analysis using 21698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21698-1-AP

  WB result of NEDD4 antibody (21698-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEDD4 transfected HeLa cells.

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  WB analysis of HT-1080 using 21698-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21698-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of C6 using 21698-1-AP

  IP result of anti-NEDD4 (IP:21698-1-AP, 4ug; Detection:21698-1-AP 1:500) with C6 cells lysate 1600ug.

• 

  IHC staining of human breast cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 21698-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NEDD4 antibody (21698-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, C6-Zellen, COS-7-Zellen, HEK-293-Zellen, HeLa-Zellen, HT-1080.Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: C6-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 55 publications below
• IHC: See 7 publications below
• IF: See 13 publications below
• IP: See 8 publications below
• CoIP: See 3 publications below

Produktinformation

21698-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA NEDD4 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEDD4 fusion protein Ag16291
• Vollständiger Name: neural precursor cell expressed, developmentally down-regulated 4
• Berechnetes Molekulargewicht: 1319 aa, 149 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC152452
• Gene symbol: NEDD4
• Gene ID (NCBI): 4734
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2 (PMID: 23545411). NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain (PMID: 35328067). NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).

Publikationen

Species	Application	Title
human,mouse	WB	Adv Sci (Weinh); OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model; Authors - Xiaomeng Song
mouse	WB	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou
human	WB	J Exp Clin Cancer Res; PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer; Authors - Liang Xu
mouse	WB	J Biomed Sci; Tudor-SN exacerbates pathological vascular remodeling by promoting the polyubiquitination of PTEN via NEDD4-1; Authors - Yichen Wu
mouse	WB	Proc Natl Acad Sci U S A; Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis.; Authors - Siming Li
human	WB	Theranostics; PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.; Authors - Xiaoxiao Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

CaX (Verified Customer) (09-01-2025)

A good antibody to probe endogenous NEDD4.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

NEDD4 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:21698-1-AP)

FH

Tongbin (Verified Customer) (08-25-2020)

This NEDD4 antibody works very well in western blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart