NF-H/NF200 Polyklonaler Antikörper

NF-H/NF200 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 21471-1-AP

Synonyme

NEFH, Neurofilament heavy polypeptide, NF200, NF-H, 200 kDa neurofilament protein

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  WB analysis of mouse brain using 21471-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21471-1-AP (NF-H antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain tissue using 21471-1-AP

  WB result of 21471-1-AP.

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  WB analysis of mouse brain using 21471-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21471-1-AP (NF-H antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21471-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21471-1-AP (NF-H antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 21471-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21471-1-AP (NF-H antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 21471-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21471-1-AP (NF-H antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of rat brain using 21471-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (21471-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 4 publications below

Produktinformation

21471-1-AP bindet in WB, IHC, IF-P, ELISA NF-H/NF200 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NF-H/NF200 fusion protein Ag15249
• Vollständiger Name: neurofilament, heavy polypeptide
• Berechnetes Molekulargewicht: 1026 aa, 112 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC073969
• Gene symbol: NF-H
• Gene ID (NCBI): 4744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd. This antibody recognize NEFH only.

Publikationen

Species	Application	Title
rat	WB,IF	Mater Today Bio; Ropivacaine microsphere-loaded electroconductive nerve dressings for long-acting analgesia and functional recovery following diabetic peripheral nerve injury; Authors - Fangguo Liang
mouse	WB	Sci Total Environ; Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.; Authors - Huanliang Liu
monkey	IF	iScience; Synthetic amyloid-β oligomers drive early pathological progression of Alzheimer's disease in nonhuman primates.; Authors - Feng Yue
monkey	WB	J Alzheimers Dis; A mass-producible macaque model displays a durable Alzheimer-like cognitive deficit and hallmark amyloid-β/tau/neurofilament light chain pathologies; Authors - Feng He
rat		Toxicol Appl Pharmacol; Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways.; Authors - Zhanjun Ma
mouse	IF	Plast Reconstr Surg Glob Open; A Novel, Sterilized Microvascular Tissue Product Improves Healing in a Murine Pressure Ulcer Model.; Authors - Jeffrey M Gimble