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NF-L Monoklonaler Antikörper

NF-L Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

5C12G4

Kat-Nr. : 60189-1-Ig

Synonyme

68 kDa neurofilament protein, NEFL, Neurofilament L, NF, NF68, NFL, NF-L

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using NF-L Monoclonal antibody (60189-1-Ig)

    WB analysis using 60189-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60189-1-Ig (NF-L antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IHC staining of rat brain using 60189-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IHC staining of mouse brain using 60189-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IHC staining of human brain using 60189-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IF Staining of mouse brain using 60189-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60189-1-Ig (NF-L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NF-L Monoclonal antibody (60189-1-Ig)

    IF Staining of rat brain using 60189-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NF-L antibody (60189-1-Ig, Clone: 5C12G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of PC-12 cells using NF-L Monoclonal antibody (60189-1-Ig)

    FC experiment of PC-12 using 60189-1-Ig

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-L (60189-1-Ig, Clone:5C12G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBPC-12-Zellen, Hausschwein-Hirngewebe, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IHCRattenhirngewebe, humanes Hirngewebe, Maushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFMaushirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in FCPC-12-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:20000-1:100000
Immunhistochemie (IHC)IHC : 1:250-1:1000
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60189-1-Ig bindet in WB, IHC, IF, FC, ELISA NF-L und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen NF-L fusion protein Ag15178
Vollständiger Name neurofilament, light polypeptide
Berechnetes Molekulargewicht 543 aa, 62 kDa
Beobachtetes Molekulargewicht 65 kDa
GenBank-ZugangsnummerBC039237
Gene symbol NEFL
Gene ID (NCBI) 4747
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL.

Protokolle

Produktspezifische Protokolle
WB protocol for NF-L antibody 60189-1-IgProtokoll herunterladen
IHC protocol for NF-L antibody 60189-1-IgProtokoll herunterladen
IF protocol for NF-L antibody 60189-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen