NEK1 Polyklonaler Antikörper

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• KD/KO Validated

NEK1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 27146-1-AP

Synonyme

EC:2.7.11.1, KIAA1901, Never in mitosis A-related kinase 1, NimA related protein kinase 1, NimA-related protein kinase 1

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  WB analysis of HeLa using 27146-1-AP

  WB result of NEK1 antibody (27146-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEK1 transfected HeLa cells.

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  WB analysis of PC-12 using 27146-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 27146-1-AP (NEK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 27146-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 27146-1-AP (NEK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-12 using 27146-1-AP

  IP result of anti-NEK1 (IP:27146-1-AP, 4ug; Detection:27146-1-AP 1:500) with PC-12 cells lysate 3080 ug.

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  IP experiment of HeLa using 27146-1-AP

  HeLa lysates prepared and IP of NEK1 performed using 1.0 μg of 27146-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human lung cancer using 27146-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27146-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27146-1-AP (NEK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27146-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NEK1 antibody (27146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, HeLa-Zellen, Maus-Pankreasgewebe
• Erfolgreiche IP: PC-12-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

27146-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NEK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEK1 fusion protein Ag25970
• Vollständiger Name: NIMA (never in mitosis gene a)-related kinase 1
• Berechnetes Molekulargewicht: 1258 aa, 143 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC114491
• Gene symbol: NEK1
• Gene ID (NCBI): 4750
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IP	Life Sci Alliance; Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases; Authors - Mateusz Gregorczyk
human	WB,IHC	Cancers (Basel); Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus; Authors - Lei Chen
human	WB	Adv Sci (Weinh); HDAC8 Enhances the Function of HIF-2α by Deacetylating ETS1 to Decrease the Sensitivity of TKIs in ccRCC; Authors - Kang Qian ; KD Validated