NEK11 Polyklonaler Antikörper

NEK11 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12710-1-AP

Synonyme

NEK11, NimA related protein kinase 11

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12710-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12710-1-AP (NEK11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12710-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12710-1-AP (NEK11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12710-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12710-1-AP (NEK11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12710-1-AP bindet in WB,ELISA NEK11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEK11 fusion protein Ag3392
• Vollständiger Name: NIMA (never in mitosis gene a)- related kinase 11
• Berechnetes Molekulargewicht: 54 kDa, 74 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC028587
• Gene symbol: NEK11
• Gene ID (NCBI): 79858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEK11(Serine/threonine-protein kinase) belongs to the NIMA family of kinases, which are involved in DNA replication and genotoxic stress responses.It controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.It has 4 isoforms produced by alternative splicing.