NELFB Polyklonaler Antikörper

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NELFB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 16418-1-AP

Synonyme

NELF-B, NELF B, Negative elongation factor B, KIAA1182, Cofactor of BRCA1

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  WB analysis using 16418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16418-1-AP (NELFB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16418-1-AP

  WB result of NELFB antibody (16418-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NELFB transfected HeLa cells.

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  WB analysis of BxPC-3 using 16418-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16418-1-AP (NELFB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 16418-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16418-1-AP (NELFB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16418-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16418-1-AP (NELFB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16418-1-AP

  IP result of anti-NELFB (IP:16418-1-AP, 3ug; Detection:16418-1-AP 1:500) with mouse brain tissue lysate 6000ug.

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  IHC staining of human urothelial carcinoma using 16418-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16418-1-AP (NELFB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 16418-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16418-1-AP (NELFB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of BxPC-3 using 16418-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 16418-1-AP (NELFB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

16418-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NELFB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NELFB fusion protein Ag9599
• Vollständiger Name: cofactor of BRCA1
• Berechnetes Molekulargewicht: 580aa,66 kDa; 475aa,54 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC011892
• Gene symbol: NELFB
• Gene ID (NCBI): 25920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.

Publikationen

Species	Application	Title
mouse	IF	Nature; The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.; Authors - Bofeng Liu
mouse	WB	Genes Dev; High constitutive activity of a broad panel of housekeeping and tissue-specific cis-regulatory elements depends on a subset of ETS proteins.; Authors - Alessia Curina
human	WB	Nat Commun; ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II; Authors - Seina Ohe
mouse	IHC	bioRxiv; RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis; Authors - Emily G Kaye
human	WB	J Biol Chem; An Essential Signaling Function of Cytoplasmic NELFB is Independent of RNA Polymerase II Pausing; Authors - Haihui Pan
mouse	IHC	Nat Commun; RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis; Authors - Emily G Kaye