Nestin Polyklonaler Antikörper
Nestin Polyklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (2)
Anwendung
WB, IHC, IF/ICC, FC (Intra), ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 19483-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Maushirngewebe, Rattenhirngewebe |
| Erfolgreiche Detektion in IF/ICC | SH-SY5Y-Zellen, U-251-Zellen |
| Erfolgreiche Detektion in FC (Intra) | SH-SY5Y-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 36 publications below |
| IHC | See 13 publications below |
| IF | See 82 publications below |
Produktinformation
19483-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Nestin und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Affe, Maus, Ratte, Zebrafisch |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | nestin |
| Berechnetes Molekulargewicht | 177 kDa |
| Beobachtetes Molekulargewicht | 170-180 kDa |
| GenBank-Zugangsnummer | NM_006617 |
| Gene symbol | Nestin |
| Gene ID (NCBI) | 10763 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Nestin antibody 19483-1-AP | Protokoll herunterladen |
| IF protocol for Nestin antibody 19483-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
ACS Nano Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury | ||
Int J Surg Stereotactically intracerebral transplantation of neural stem cells for ischemic stroke attenuated inflammatory responses and promoted neurogenesis: an experimental study with monkeys | ||
J Biomed Sci Genetic and pharmacologic p32-inhibition rescue CHCHD2-linked Parkinson's disease phenotypes in vivo and in cell models | ||
Neuro Oncol TRIM24 Promotes Stemness and Invasiveness of Glioblastoma Cells via Activating SOX2 Expression. | ||
Redox Biol MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway. | ||
Acta Biomater Induced pluripotent stem cell-derived neural stem cells promote bone formation in mice with calvarial defects |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Udesh (Verified Customer) (08-12-2025) | Worked well for both IF and western
|
FH Reyes (Verified Customer) (03-18-2024) | I tested nestin (in red) in human brain cortical sections obtained from pediatric patients, and the result was inconclusive, as it looks like ther is a halo around my DAPI, and there is no clear nestin marking in any cell type in the tissue.
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FH Udesh (Verified Customer) (02-24-2023) | Nestin in the upper lane Worked well at 1:1000 in 5% BSA, O/N, 4 degrees
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FH Balawant (Verified Customer) (08-23-2022) | this antibody is excellent for westernblot
|
FH Silvia (Verified Customer) (09-30-2021) | Immunofluorescence (1:200) works well on NPC
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