Nestin Polyklonaler Antikörper

Nestin Polyklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(119)

Kat-Nr. : 19483-1-AP

Synonyme

NES



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IF/ICCSH-SY5Y-Zellen, U-251-Zellen
Erfolgreiche Detektion in FC (Intra)SH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

19483-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Nestin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name nestin
Berechnetes Molekulargewicht 177 kDa
Beobachtetes Molekulargewicht 170-180 kDa
GenBank-ZugangsnummerNM_006617
Gene symbol Nestin
Gene ID (NCBI) 10763
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Nestin antibody 19483-1-APProtokoll herunterladen
IF protocol for Nestin antibody 19483-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratIF

ACS Nano

Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury

Authors - Weidong Wu
monkeyIF

Int J Surg

Stereotactically intracerebral transplantation of neural stem cells for ischemic stroke attenuated inflammatory responses and promoted neurogenesis: an experimental study with monkeys

Authors - Yi-Fan Liu
IF

J Biomed Sci

Genetic and pharmacologic p32-inhibition rescue CHCHD2-linked Parkinson's disease phenotypes in vivo and in cell models

Authors - Murni Tio
humanWB,IF

Neuro Oncol

TRIM24 Promotes Stemness and Invasiveness of Glioblastoma Cells via Activating SOX2 Expression.

Authors - Lu-Hua Zhang
mouse,humanIF

Redox Biol

MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway.

Authors - Shanshan Ma
mouseIF

Acta Biomater

Induced pluripotent stem cell-derived neural stem cells promote bone formation in mice with calvarial defects

Authors - Yukari Shiwaku

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Udesh (Verified Customer) (08-12-2025)

Worked well for both IF and western

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MSCs
FH

Reyes (Verified Customer) (03-18-2024)

I tested nestin (in red) in human brain cortical sections obtained from pediatric patients, and the result was inconclusive, as it looks like ther is a halo around my DAPI, and there is no clear nestin marking in any cell type in the tissue.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
Nestin Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:19483-1-AP)
FH

Udesh (Verified Customer) (02-24-2023)

Nestin in the upper lane Worked well at 1:1000 in 5% BSA, O/N, 4 degrees

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS Osteosarcoma cells
Nestin Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:19483-1-AP)
FH

Balawant (Verified Customer) (08-23-2022)

this antibody is excellent for westernblot

  • Applications: Western Blot
  • Cell Tissue Type: colon tissue and colon cell line
FH

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence (1:200) works well on NPC

  • Applications: Immunofluorescence