Nestin Polyklonaler Antikörper

Nestin Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

74

Rezensionen (3)

Kat-Nr. : 19483-1-AP

Synonyme

NES, Nestin

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Galerie der Validierungsdaten

• 

  WB analysis using 19483-1-AP

  Mouse brain lysates were subjected to SDS PAGE followed by western blot with 19483-1-AP (Nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 19483-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19483-1-AP (NES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human gliomas using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human kidney using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of mouse brain using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of rat brain using 19483-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Nestin antibody (19483-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U-251 using 19483-1-AP

  Immunofluorescent analysis of U-251 cells using 19483-1-AP (Nestin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 19483-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 19483-1-AP (Nestin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with sox2 (66411-1-Ig, red).

• 

  IF Staining of SH-SY5Y using 19483-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 19483-1-AP (Nestin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 19483-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 19483-1-AP (Nestin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Gliomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe, Maushirngewebe, SH-SY5Y-Zellen, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 27 publications below
• IHC: See 9 publications below
• IF: See 48 publications below
• FC: See 1 publications below

Produktinformation

19483-1-AP bindet in WB, IHC, IF, FC, ELISA Nestin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nestin
• Berechnetes Molekulargewicht: 177 kDa
• Beobachtetes Molekulargewicht: 170-180 kDa
• GenBank-Zugangsnummer: NM_006617
• Gene symbol: NES
• Gene ID (NCBI): 10763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).

Publikationen

Species	Application	Title
rat	IF	ACS Nano; Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury; Authors - Weidong Wu
human	WB,IF	Neuro Oncol; TRIM24 Promotes Stemness and Invasiveness of Glioblastoma Cells via Activating SOX2 Expression.; Authors - Lu-Hua Zhang
human,mouse	IF	Redox Biol; MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway.; Authors - Shanshan Ma
rat	IF	Neuropsychopharmacology; Exosomes from patients with major depression cause depressive-like behaviors in mice with involvement of miR-139-5p-regulated neurogenesis.; Authors - Ze-Xu Wei
rat	IF	J Mater Chem B; Integration of genetically engineered virus nanofibers and fibrin to form injectable fibrous neuron-rich hydrogels and enable neural differentiation; Authors - Yingfan Chen
human	WB	PLoS Pathog; Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells; Authors - Xian-Zhang Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (02-24-2023)

Nestin in the upper lane Worked well at 1:1000 in 5% BSA, O/N, 4 degrees

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS Osteosarcoma cells

Nestin Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:19483-1-AP)

FH

Balawant (Verified Customer) (08-23-2022)

this antibody is excellent for westernblot

• Applications: Western Blot
• Cell Tissue Type: colon tissue and colon cell line

FH

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence (1:200) works well on NPC

• Applications: Immunofluorescence

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