NEU1 Monoklonaler Antikörper

NEU1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

1F6D1

Kat-Nr. : 67032-1-Ig

Synonyme

1F6D1, Acetylneuraminyl hydrolase, EC:3.2.1.18, G9 sialidase, Lysosomal sialidase

---

• 

  WB analysis of PC-12 using 67032-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67032-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 67032-1-Ig

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 67032-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 67032-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67032-1-Ig (NEU1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 67032-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 67032-1-Ig (NEU1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, Mauslebergewebe, Hausschwein-Lebergewebe, Rattenlebergewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

67032-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA NEU1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NEU1 fusion protein Ag27945
• Vollständiger Name: sialidase 1 (lysosomal sialidase)
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa
• GenBank-Zugangsnummer: BC011900
• Gene symbol: NEU1
• Gene ID (NCBI): 4758
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEU1 (lysosomal sialidase) encodes a kind of lysosomal enzyme that could cleave terminal silic acids from glycoproteins or glycolipids to regulate various biological processes by conformational change (PMID:28130415). In the lysosome, NEU1 is one member of a heterotrimeric complex, the others are beta-galactosidase and cathepsin A. The NEU1 is widely expressed in mammalian tissues and involved in lysosomal storage disorder sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells (PMID:19075514). NUE1 also expresses on the plasma membrane where it modulates several signalling molecules about inflamation, exocytosis, phagocytosis, cell adhesion and proliferation (PMID:21928149). Addition, it is reported that mutation in NEU1 of human could lead to sialidosis (PMID:14517945).

Publikationen

Species	Application	Title
human	WB	Eur Rev Med Pharmacol Sci; Long non-coding RNA TOB1-AS1 modulates cell proliferation, apoptosis, migration and invasion through miR-23a/NEU1 axis via Wnt/b-catenin pathway in gastric cancer.; Authors - K Jiang
human	IF	Br J Pharmacol; Neuraminidase is a host-directed approach to regulate neutrophil responses in sepsis and COVID-19; Authors - Rodrigo de Oliveira Formiga
mouse,human	WB,IP,IF	Nat Commun; Neuraminidase 1 promotes renal fibrosis development in male mice; Authors - Qian-Qian Chen
human	WB,IF	Biomed Pharmacother; Oseltamivir enhances 5-FU sensitivity in esophageal squamous carcinoma with high SPNS1; Authors - Xin Yang
mouse	WB	Virology; H1N1 swine influenza viruses upregulate NEU1 expression through histone H3 acetylation regulated by HDAC2; Authors - Qiuyi Zhao
human	IHC	Research (Wash D C); Proteogenomic Characterization of High-Grade Lung Neuroendocrine Carcinoma Deciphers Molecular Diversity and Potential Biomarkers of Different Histological Subtypes in Chinese Population; Authors - Zicheng Zhang