NEU1 Monoklonaler Antikörper

NEU1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1F6D1

Kat-Nr. : 67032-1-Ig

Synonyme

Acetylneuraminyl hydrolase, FLJ93471, G9 sialidase, Lysosomal sialidase, N acetyl alpha neuraminidase 1, NANH, NEU, NEU1, NEURAMINIDASE 1, SIAL1, Sialidase 1

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Galerie der Validierungsdaten

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  WB analysis of PC-12 using 67032-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 67032-1-Ig

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 67032-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67032-1-Ig (NEU1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67032-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67032-1-Ig (NEU1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 67032-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using 67032-1-Ig (NEU1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, Hausschwein-Lebergewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

67032-1-Ig bindet in WB, IP, IHC, IF, ELISA NEU1 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NEU1 fusion protein Ag27945
• Vollständiger Name: sialidase 1 (lysosomal sialidase)
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa
• GenBank-Zugangsnummer: BC011900
• Gene symbol: NEU1
• Gene ID (NCBI): 4758
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEU1 (lysosomal sialidase) encodes a kind of lysosomal enzyme that could cleave terminal silic acids from glycoproteins or glycolipids to regulate various biological processes by conformational change (PMID:28130415). In the lysosome, NEU1 is one member of a heterotrimeric complex, the others are beta-galactosidase and cathepsin A. The NEU1 is widely expressed in mammalian tissues and involved in lysosomal storage disorder sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells (PMID:19075514). NUE1 also expresses on the plasma membrane where it modulates several signalling molecules about inflamation, exocytosis, phagocytosis, cell adhesion and proliferation (PMID:21928149). Addition, it is reported that mutation in NEU1 of human could lead to sialidosis (PMID:14517945).

Publikationen

Species	Application	Title
human	WB	Eur Rev Med Pharmacol Sci; Long non-coding RNA TOB1-AS1 modulates cell proliferation, apoptosis, migration and invasion through miR-23a/NEU1 axis via Wnt/b-catenin pathway in gastric cancer.; Authors - K Jiang
human	IF	Br J Pharmacol; Neuraminidase is a host-directed approach to regulate neutrophil responses in sepsis and COVID-19; Authors - Rodrigo de Oliveira Formiga
mouse,human	WB,IF,IP	Nat Commun; Neuraminidase 1 promotes renal fibrosis development in male mice; Authors - Qian-Qian Chen