NFATC2 Polyklonaler Antikörper

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• KD/KO Validated

NFATC2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 22023-1-AP

Synonyme

NF ATc2, NF ATp, NFAT pre existing subunit, NFAT1, NFATC2, NFATP

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 22023-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22023-1-AP (NFATC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 22023-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 22023-1-AP (NFATC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 22023-1-AP

  IP Result of anti-NFATC2 (IP:22023-1-AP, 4ug; Detection:22023-1-AP 1:1000) with Jurkat cells lysate 3200ug.

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  IHC staining of human breast cancer using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 22023-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NFATC2 antibody (22023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 22023-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human NFATC2 (22023-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U-251-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Produktinformation

22023-1-AP bindet in WB, IP, IHC, IF, FC, ELISA NFATC2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFATC2 fusion protein Ag16849
• Vollständiger Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
• Berechnetes Molekulargewicht: 925 aa, 100 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC136418
• Gene symbol: NFATC2
• Gene ID (NCBI): 4773
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.

Publikationen

Species	Application	Title
mouse	WB, IF	Biomaterials; Targeted regulation of lymphocytic ER stress response with an overall immunosuppression to alleviate allograft rejection.; Authors - Yingying Shi
human	WB,IF	Breast Cancer Res; Calcium-sensing stromal interaction molecule 2 upregulates nuclear factor of activated T cells 1 and transforming growth factor-β signaling to promote breast cancer metastasis.; Authors - Yutian Miao ; KD Validated
human	WB,IHC	Cell Mol Gastroenterol Hepatol; ANKRD22 Drives Rapid Proliferation of Lgr5+ Cells and Acts as a Promising Therapeutic Target in Gastric Mucosal Injury.; Authors - Jingwen Liu
	IF	Mucosal Immunol; IL-33/ST2/IL-9/IL-9R signaling disrupts ocular surface barrier in allergic inflammation.; Authors - Jiaoyue Hu
mouse	WB	J Neuroinflammation; Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model; Authors - Yitian Lu
mouse	WB	CNS Neurosci Ther; miR-26a-5p alleviates CFA-induced chronic inflammatory hyperalgesia through Wnt5a/CaMKII/NFAT signaling in mice; Authors - Yitian Lu