NFKB2,p100 Rekombinanter Antikörper

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• KD/KO Validated

NFKB2,p100 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241126C9

Kat-Nr. : 84022-5-RR

Synonyme

NFKB2, 241126C9, DNA binding factor KBF2, DNA-binding factor KBF2, H2TF1

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  WB analysis using 84022-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84022-5-RR (NFKB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 84022-5-RR

  WB result of NFKB2,p100 antibody (84022-5-RR; 1:60000; room temperature for 1.5 hours) with wild-type and sg-NFKB2 transfected HAP1 cells.

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  FC experiment of HeLa using 84022-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NFKB2,p100 Recombinant antibody (84022-5-RR, Clone:241126C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A431 using 84022-5-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug NFKB2,p100 Recombinant antibody (84022-5-RR, Clone:241126C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84022-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84022-5-RR against Human NFKB2,p100 were performed. The affinity constant is 0.109 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84022-5-RR bindet in WB, FC (Intra), ELISA NFKB2,p100 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NFKB2,p100 fusion protein Ag0662
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC002844
• Gene symbol: NFKB2
• Gene ID (NCBI): 4791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.