IkB Alpha Polyklonaler Antikörper

IkB Alpha Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(611)

Kat-Nr. : 10268-1-AP

Synonyme

IKBA, IκB α, NFKBIA, I kappa B alpha, IkappaBalpha



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Hausschwein-Lebergewebe
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Magengewebe, humanes Nierengewebe, humanes Prostatakarzinomgewebe, Mausnierengewebe, Ratten-Magengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen, A431-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:1500-1:6000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10268-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA IkB Alpha und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Huhn, Maus, Ratte, Rind
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen IkB Alpha fusion protein Ag0345
Vollständiger Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Berechnetes Molekulargewicht 36 kDa
Beobachtetes Molekulargewicht 36 kDa
GenBank-ZugangsnummerBC002601
Gene symbol IkB Alpha
Gene ID (NCBI) 4792
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IkB Alpha antibody 10268-1-APProtokoll herunterladen
IHC protocol for IkB Alpha antibody 10268-1-APProtokoll herunterladenl
IF protocol for IkB Alpha antibody 10268-1-APProtokoll herunterladen
IP protocol for IkB Alpha antibody 10268-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Cell Metab

Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer

Authors - Yu Jiang
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
humanWB

Cancer Commun (Lond)

Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer

Authors - Xingbin Hu
mouseWB

Mol Cell

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

Authors - Chuanlong Wang
mouseWB

Adv Sci (Weinh)

Reprogramming Lung Redox Homeostasis by NIR Driven Ultra-Small Pd Loaded Covalent Organic Framework Inhibits NF-κB Pathway for Acute Lung Injury Immunotherapy

Authors - Doudou Lei
humanWB,IF

Nucleic Acids Res

p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway

Authors - Xinxing Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

P (Verified Customer) (07-15-2025)

Used for caco2 cells and animal colon tissue

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1 to 1000
FH

SONAM (Verified Customer) (10-04-2023)

Antibody worked well. No nonspecific band was observed.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: cancer cells
FH

CHAO (Verified Customer) (01-30-2020)

Weak bands for the heart lysates at basal state.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
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