IkB Alpha Polyklonaler Antikörper
IkB Alpha Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
Hausschwein, human, Maus, Ratte und mehr (2)
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10268-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Hausschwein-Lebergewebe |
| Erfolgreiche IP | HeLa-Zellen |
| Erfolgreiche Detektion in IHC | humanes Magengewebe, humanes Nierengewebe, humanes Prostatakarzinomgewebe, Mausnierengewebe, Ratten-Magengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen, A431-Zellen |
| Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:1500-1:6000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 588 publications below |
| IHC | See 15 publications below |
| IF | See 9 publications below |
| CoIP | See 2 publications below |
Produktinformation
10268-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA IkB Alpha und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Maus, Ratte, Rind |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | IkB Alpha fusion protein Ag0345 |
| Vollständiger Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| Berechnetes Molekulargewicht | 36 kDa |
| Beobachtetes Molekulargewicht | 36 kDa |
| GenBank-Zugangsnummer | BC002601 |
| Gene symbol | IkB Alpha |
| Gene ID (NCBI) | 4792 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for IkB Alpha antibody 10268-1-AP | Protokoll herunterladen |
| IHC protocol for IkB Alpha antibody 10268-1-AP | Protokoll herunterladenl |
| IF protocol for IkB Alpha antibody 10268-1-AP | Protokoll herunterladen |
| IP protocol for IkB Alpha antibody 10268-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cell Metab Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer | ||
Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation | ||
Adv Sci (Weinh) Reprogramming Lung Redox Homeostasis by NIR Driven Ultra-Small Pd Loaded Covalent Organic Framework Inhibits NF-κB Pathway for Acute Lung Injury Immunotherapy | ||
Nucleic Acids Res p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH P (Verified Customer) (07-15-2025) | Used for caco2 cells and animal colon tissue
|
FH SONAM (Verified Customer) (10-04-2023) | Antibody worked well. No nonspecific band was observed.
|
FH CHAO (Verified Customer) (01-30-2020) | Weak bands for the heart lysates at basal state.
|























