NHP2L1 Polyklonaler Antikörper

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• KD/KO Validated

NHP2L1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 15802-1-AP

Synonyme

SNU13, OTK27, NHP2-like protein 1, N-terminally processed, NHP2-like protein 1, hSNU13

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  WB analysis using 15802-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15802-1-AP (NHP2L1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15802-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15802-1-AP (NHP2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 15802-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15802-1-AP (NHP2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15802-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15802-1-AP (NHP2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 15802-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15802-1-AP (NHP2L1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 15802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15802-1-AP (NHP2L1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 15802-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15802-1-AP (NHP2L1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 15802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15802-1-AP (NHP2L1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A431 using 15802-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NHP2L1 antibody (15802-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Jurkat-Zellen, MCF-7-Zellen, Maushirngewebe, Maus-Pankreasgewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

15802-1-AP bindet in WB, IHC, IF/ICC, ELISA NHP2L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NHP2L1 fusion protein Ag8532
• Vollständiger Name: NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 128 aa, 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC005358
• Gene symbol: NHP2L1
• Gene ID (NCBI): 4809
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NHP2L1, belongs to the ribosomal protein L7Ae family, is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs, and is required for subsequent recruitment of PRP31 and activation of the spliceosomal complex [PMID: 17412961]. It binds to the 5'-stem-loop of U4 snRNA and may have a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding [PMID: 10545122].

Publikationen

Species	Application	Title
human	WB	Mol Cell; Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus.; Authors - Run-Wen Yao ; KO Validated
human	IF	Mol Cell; Condensates induced by transcription inhibition localize active chromatin to nucleoli.; Authors - Takaaki Yasuhara
human	WB	Mol Cell; LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.; Authors - Xin Wang
	WB	Nucleic Acids Res; NUFIP and the HSP90/R2TP chaperone bind the SMN complex and facilitate assembly of U4-specific proteins.; Authors - Jonathan Bizarro
mouse	WB	Biochim Biophys Acta Proteins Proteom; Phosphoproteomic response of cardiac endothelial cells to ischemia and ultrasound.; Authors - Uchenna Emechebe