NINJ2 Monoklonaler Antikörper

NINJ2 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

5C5B2

Kat-Nr. : CL594-66202

Synonyme

Ninjurin-2, Nerve injury-induced protein 2, 5C5B2

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  FC experiment of U2OS using CL594-66202

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug Coralite®594 Ninj2 Monoclonal Antibody (CL594-66202, Clone:5C5B2)(red), or 0.4 ug CoraLite®594 Mouse IgG2b isotype control (11B8C4) (CL594-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66202 bindet in FC (Intra) NINJ2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NINJ2 fusion protein Ag5343
• Vollständiger Name: ninjurin 2
• Berechnetes Molekulargewicht: 142 aa, 16 kDa
• GenBank-Zugangsnummer: BC057766
• Gene symbol: NINJ2
• Gene ID (NCBI): 4815
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NINJ2 (Ninjurin2) is a member of the ninjurin family of adhesion molecules which mediate cell-to-cell and cell-to-extracellular matrix interactions during development, differentiation, and regeneration of the peripheral nervous system. The gene encodes NINJ2 is located on chromosome 12p13. NINJ2 mRNA is widely expressed in adult human tissues, with highest level in bone marrow, followed by peripheral leukocytes, lung, and lymph nodes. In the peripheral nervous system, NINJ2 is expressed constitutively in mature sensory and enteric neurons. The expression of NINJ2 is upregulated after nerve injury in Schwann cells, suggesting that it may promote nerve regeneration. It may also play an important role in the pathogenesis of inflammatory disorder. (PMID: 10627596)