NIPBL Polyklonaler Antikörper

NIPBL Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 18792-1-AP

Synonyme

CDLS, CDLS1, Delangin, IDN3, IDN3 B, NIPBL, Nipped B homolog (Drosophila), Nipped B like protein, SCC2 homolog

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 18792-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18792-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 18792-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18792-1-AP (NIPBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

18792-1-AP bindet in WB, IF, ChIP, ELISA NIPBL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NIPBL fusion protein Ag5663
• Vollständiger Name: Nipped-B homolog (Drosophila)
• Berechnetes Molekulargewicht: 2804 aa, 316 kDa
• Beobachtetes Molekulargewicht: 300 kDa
• GenBank-Zugangsnummer: BC033847
• Gene symbol: NIPBL
• Gene ID (NCBI): 25836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NIPBL, also named as Nipped-B-like protein, is a 28-4 amino acid protein, which Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains and belongs to the SCC2/Nipped-B family. NIPBL probably plays a structural role in chromatin and Involve in sister chromatid cohesion, possibly by interacting with the cohesin complex. endogenous full length NIPBL which is estimated to be about 300kDa led us to believe that the NIPBL protein was deteriorated or lost by the standard protein extraction and blotting techniques.

Publikationen

Species	Application	Title
mouse	ChIP	Cell; Lineage-specific 3D genome organization is assembled at multiple scales by IKAROS; Authors - Yeguang Hu
human	WB	Nat Commun; C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.; Authors - Dan Liu
mouse	ChIP	Nat Immunol; BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin; Authors - Malay Mandal