NLRX1 Polyklonaler Antikörper

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• KD/KO Validated

NLRX1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(24)

Kat-Nr. : 17215-1-AP

Synonyme

NLR family member X1, NLRX 1, NOD5, NOD9, Nucleotide-binding oligomerization domain protein 5

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  WB analysis using 17215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17215-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17215-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17215-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 17215-1-AP

  IP result of anti-NLRX1 (IP:17215-1-AP, 3ug; Detection:17215-1-AP 1:800) with mouse heart tissue lysate 3520ug.

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  IHC staining of human heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17215-1-AP (NLRX1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17215-1-AP (NLRX1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:50.

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  IF Staining of A549 using 17215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NLRX1 antibody (17215-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 17215-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NLRX1 (17215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, Maus-Kolongewebe, Mausherzgewebe, Maus-Skelettmuskelgewebe, Ratten-Kolongewebe, Rattenherzgewebe, THP-1-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Lebergewebe, humanes Skelettmuskelgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

17215-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA NLRX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NLRX1 fusion protein Ag11000
• Vollständiger Name: NLR family member X1
• Berechnetes Molekulargewicht: 975 aa, 108 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC013199
• Gene symbol: NLRX1
• Gene ID (NCBI): 79671
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NLRX1 (Nucleotide-binding oligomerization domain, leucine-rich repeat containing X1) is a member of the NOD-like receptor (NLR) family and is unique among NLRs due to its localization to the mitochondrial matrix. NLRX1 is a negative regulator of innate immunity, particularly in viral infections. It interacts with the mitochondrial antiviral signaling protein (MAVS) to attenuate antiviral responses. NLRX1 has been implicated in various diseases, including multiple sclerosis, colorectal cancer, and ischemia-reperfusion injury. Its role in controlling inflammation and mitochondrial function makes it a potential therapeutic target.

Publikationen

Species	Application	Title
mouse	WB	Nature; Mitochondrial DNA stress primes the antiviral innate immune response.; Authors - A Phillip West
human	WB	Mol Cell; Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1.; Authors - Samuel A Killackey ; KD Validated
rat	WB,IF	Cell Death Differ; NLRX1 mediated impaired microglial phagocytosis of NETs in cerebral ischemia and reperfusion injury; Authors - Jialing Peng
human	WB,IHC	J Exp Med; NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity.; Authors - Geurt Stokman ; KO Validated
mouse,human	WB	PLoS Pathog; PMID: 28542569; Authors - Jae-Hoon Kim
human	WB	Front Immunol; PMID: 30344524; Authors - Tünde Fekete