NODAL Polyklonaler Antikörper

NODAL Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27430-1-AP

Synonyme

nodal homolog (mouse), Nodal homolog

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  WB analysis of HeLa using 27430-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27430-1-AP (NODAL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 27430-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27430-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 27430-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 27430-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 27430-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NODAL antibody (27430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 27430-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NODAL antibody (27430-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HEK-293T using 27430-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human NODAL (27430-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27430-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA NODAL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NODAL fusion protein Ag26729
• Vollständiger Name: nodal homolog (mouse)
• Berechnetes Molekulargewicht: 347 aa, 40 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC104976
• Gene symbol: NODAL
• Gene ID (NCBI): 4838
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nodal is a member of the transforming growth factor-β (TGF-β) superfamily that plays critical roles during embryogenesis. Nodal also regulates cell proliferation, apoptosis, and invasion in cancer cells. However, it appears to exert both tumor-suppressing and tumor-promoting effects, depending on the cell type (PMID: 22645523). Nodal signals through ALK7 receptor to inhibit proliferation and to induce apoptosis in ovarian cancer cell lines and breast cancer cell lines (PMID: 15531507, PMID: 21383881).