NPL Polyklonaler Antikörper

NPL Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16715-1-AP

Synonyme

N-acetylneuraminate pyruvate-lyase, N-acetylneuraminate lyase, N acetylneuraminate lyase, EC:4.1.3.3, C1orf13

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  WB analysis using 16715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16715-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 16715-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 16715-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16715-1-AP (NPL antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 16715-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NPL antibody (16715-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausnierengewebe, Raji-Zellen, Rattenhirngewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16715-1-AP bindet in WB, IHC, IF/ICC, ELISA NPL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NPL fusion protein Ag10137
• Vollständiger Name: N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)
• Berechnetes Molekulargewicht: 27 kDa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC058003
• Gene symbol: NPL
• Gene ID (NCBI): 80896
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The N-acetylneuraminate lyase(NPL) from human (hNAL) which consists of 320 amino acids, has a molecular mass of 35.2 kDa and it catalyses the reversible aldol reaction of sialic acid, and has been extensively used in the synthesis of sialic acids and its analogues(PMID:19057931). The enzyme is widely distributed and are present in numerous pro- and eukaryotic cells, in which they are localized only in the cytosol(PMID:12453637). It belongs to the DHDPS family and has 5 isoforms produced by alternative splicing.